Probing the conformations of eight cloned-DNA dodecamers; CGCGAATTCGCG, CGCGTTAACGCG, CGCGTATACGCG, CGCGATATCGCG, CGCAAATTTGCG, CGCTTTAAAGCG, CGCGGATCCGCG and CGCGGTACCGCG
Probing the conformations of eight cloned-DNA dodecamers; CGCGAATTCGCG, CGCGTTAACGCG, CGCGTATACGCG, CGCGATATCGCG, CGCAAATTTGCG, CGCTTTAAAGCG, CGCGGATCCGCG and CGCGGTACCGCG
The self complementary DNA dodecamers d(CGCGAATTCGCG), d(CGCGTTAACGCG), d(CGCGTATACGCG), d(CGCGATATcGcG), d(CGCAAATTTGCG), d(CGCTTTAAAGCG), d(CGCGGATCCGCG) and d(CGCGGTACCGCG) have been cloned into the Smal site of plasmid pUC19. Radiolabelled polylinker fragments containing these inserts have been digested with nucleases and chemical agents, probing the structure of the central AT base pairs. The sequences AATT and AAATTT are relatively resistant to digestion by DNase I, micrococcal nuclease and hydroxyl radicals, consistent with the suggestion that they possess a narrow minor groove. Nuclease digestion of TTAA is much more even, and comparable to that at mixed sequence DNA. TpA steps in ATAT, TATA and GTAC are cut less well by DNAse I than In TTAA. DNasel cleavage of surrounding bases, especially CpG is strongly influenced by the nature of the central sequence.
6487-6493
Fox, Keith R.
9da5debc-4e45-473e-ab8c-550d1104659f
25 December 1992
Fox, Keith R.
9da5debc-4e45-473e-ab8c-550d1104659f
Fox, Keith R.
(1992)
Probing the conformations of eight cloned-DNA dodecamers; CGCGAATTCGCG, CGCGTTAACGCG, CGCGTATACGCG, CGCGATATCGCG, CGCAAATTTGCG, CGCTTTAAAGCG, CGCGGATCCGCG and CGCGGTACCGCG.
Nucleic Acids Research, 20 (24), .
(doi:10.1093/nar/20.24.6487).
Abstract
The self complementary DNA dodecamers d(CGCGAATTCGCG), d(CGCGTTAACGCG), d(CGCGTATACGCG), d(CGCGATATcGcG), d(CGCAAATTTGCG), d(CGCTTTAAAGCG), d(CGCGGATCCGCG) and d(CGCGGTACCGCG) have been cloned into the Smal site of plasmid pUC19. Radiolabelled polylinker fragments containing these inserts have been digested with nucleases and chemical agents, probing the structure of the central AT base pairs. The sequences AATT and AAATTT are relatively resistant to digestion by DNase I, micrococcal nuclease and hydroxyl radicals, consistent with the suggestion that they possess a narrow minor groove. Nuclease digestion of TTAA is much more even, and comparable to that at mixed sequence DNA. TpA steps in ATAT, TATA and GTAC are cut less well by DNAse I than In TTAA. DNasel cleavage of surrounding bases, especially CpG is strongly influenced by the nature of the central sequence.
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Published date: 25 December 1992
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Funding Information: this work was supported by grants from the Cancer Research Campaign and the Science and Engineering Research Council. K.R.F. is a Lister Institute Research Fellow.
Identifiers
Local EPrints ID: 475849
URI: http://eprints.soton.ac.uk/id/eprint/475849
ISSN: 0305-1048
PURE UUID: f41fd956-a82e-401d-928d-01930fadec10
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Date deposited: 29 Mar 2023 16:45
Last modified: 18 Mar 2024 02:32
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