Extension of DNA triple helix formation to a neighbouring (AT)n site
Extension of DNA triple helix formation to a neighbouring (AT)n site
We have used DNase I footprinting to examine the formation of intermolecular triple helices at a fragment containing the target sequence A11(AT)6 · (AT)6T11, using oligonucleotides designed to form parallel T · AT and G · TA triplets. We find that, although (TG)6 does not form a complex with (AT)6 · (AT)6, T11(TG)6 forms a stable structure producing a clear footprint which includes the (AT)6 portion of the target site. This complex is not formed in the presence of magnesium, but can be stabilised by either manganese or a triplex-binding ligand.
DNA sequence recognition, DNA triple helix, G TA triplet
21-25
Chandler, Simon P.
839d7f59-0eb0-4fc5-accf-6ff2bc08b243
Fox, Keith R.
9da5debc-4e45-473e-ab8c-550d1104659f
20 February 1995
Chandler, Simon P.
839d7f59-0eb0-4fc5-accf-6ff2bc08b243
Fox, Keith R.
9da5debc-4e45-473e-ab8c-550d1104659f
Chandler, Simon P. and Fox, Keith R.
(1995)
Extension of DNA triple helix formation to a neighbouring (AT)n site.
FEBS Letters, 360 (1), .
(doi:10.1016/0014-5793(95)00069-L).
Abstract
We have used DNase I footprinting to examine the formation of intermolecular triple helices at a fragment containing the target sequence A11(AT)6 · (AT)6T11, using oligonucleotides designed to form parallel T · AT and G · TA triplets. We find that, although (TG)6 does not form a complex with (AT)6 · (AT)6, T11(TG)6 forms a stable structure producing a clear footprint which includes the (AT)6 portion of the target site. This complex is not formed in the presence of magnesium, but can be stabilised by either manganese or a triplex-binding ligand.
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Published date: 20 February 1995
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Funding Information:
Acknowledgements: This work was supported by grants from the Cancer Research Campaign and the Medical Research Council.
Keywords:
DNA sequence recognition, DNA triple helix, G TA triplet
Identifiers
Local EPrints ID: 475852
URI: http://eprints.soton.ac.uk/id/eprint/475852
ISSN: 0014-5793
PURE UUID: a5fda96a-8c3e-4a88-9318-88c4cee6a618
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Date deposited: 29 Mar 2023 16:45
Last modified: 18 Mar 2024 02:32
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Author:
Simon P. Chandler
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