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Bifunctional Intercalator [N-MeCys3,N-MeCys7]TANDEM Binds to the Dinucleotide TpA

Bifunctional Intercalator [N-MeCys3,N-MeCys7]TANDEM Binds to the Dinucleotide TpA
Bifunctional Intercalator [N-MeCys3,N-MeCys7]TANDEM Binds to the Dinucleotide TpA

The binding of [N-MeCys3,N-MeCys7]TANDEM has been examined by DNase I footprinting and diethyl pyrocarbonate modification of several synthetic DNA fragments containing AT-rich regions. DNase I footprinting reveals that at low concentrations the ligand binds preferentially to the center of (AT)n regions. A fragment containing the tetranucleotide AATT was unaffected by the ligand. Diethyl pyrocarbonate modification of several fragments containing blocks of (AT)n revealed a pattern in which alternate adenines were rendered more reactive in the presence of the ligand. These reactive adenines were staggered across the two DNA strands in the 3ʹ-direction, consistent with ligand binding to the dinucleotide TpA. In sequences of the type (TAA)n·(TTA)n, binding of [N-MeCys3,N-MeCys7]TANDEM resulted in strong modification of the second adenine in the sequence TAA, i.e., the base on the 3ʹ-side of the ligand binding site. Data for binding to (AT)n are best explained by suggesting that the adenines sandwiched between the quinoxaline chromophores are rendered most reactive to diethyl pyrocarbonate.

0006-2960
6246-6253
Waterloh, Kerstin
25acfef4-af28-43e0-b0a7-b9f237569c2d
Fox, Keith R.
9da5debc-4e45-473e-ab8c-550d1104659f
Olsen, Richard K.
cc91281f-1f15-494e-aef0-62f564a36e31
Waterloh, Kerstin
25acfef4-af28-43e0-b0a7-b9f237569c2d
Fox, Keith R.
9da5debc-4e45-473e-ab8c-550d1104659f
Olsen, Richard K.
cc91281f-1f15-494e-aef0-62f564a36e31

Waterloh, Kerstin, Fox, Keith R. and Olsen, Richard K. (1992) Bifunctional Intercalator [N-MeCys3,N-MeCys7]TANDEM Binds to the Dinucleotide TpA. Biochemistry, 31 (27), 6246-6253. (doi:10.1021/bi00142a012).

Record type: Article

Abstract

The binding of [N-MeCys3,N-MeCys7]TANDEM has been examined by DNase I footprinting and diethyl pyrocarbonate modification of several synthetic DNA fragments containing AT-rich regions. DNase I footprinting reveals that at low concentrations the ligand binds preferentially to the center of (AT)n regions. A fragment containing the tetranucleotide AATT was unaffected by the ligand. Diethyl pyrocarbonate modification of several fragments containing blocks of (AT)n revealed a pattern in which alternate adenines were rendered more reactive in the presence of the ligand. These reactive adenines were staggered across the two DNA strands in the 3ʹ-direction, consistent with ligand binding to the dinucleotide TpA. In sequences of the type (TAA)n·(TTA)n, binding of [N-MeCys3,N-MeCys7]TANDEM resulted in strong modification of the second adenine in the sequence TAA, i.e., the base on the 3ʹ-side of the ligand binding site. Data for binding to (AT)n are best explained by suggesting that the adenines sandwiched between the quinoxaline chromophores are rendered most reactive to diethyl pyrocarbonate.

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Published date: 1 February 1992

Identifiers

Local EPrints ID: 475856
URI: http://eprints.soton.ac.uk/id/eprint/475856
ISSN: 0006-2960
PURE UUID: 6fbb1182-2fa5-4c99-9c01-18dd4cc28985
ORCID for Keith R. Fox: ORCID iD orcid.org/0000-0002-2925-7315

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Date deposited: 29 Mar 2023 16:46
Last modified: 17 Mar 2024 02:34

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Contributors

Author: Kerstin Waterloh
Author: Keith R. Fox ORCID iD
Author: Richard K. Olsen

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