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Kinetic studies on the formation of acridine-linked DNA triple helices

Kinetic studies on the formation of acridine-linked DNA triple helices
Kinetic studies on the formation of acridine-linked DNA triple helices

We have used DNase I footprinting to measure the rate of intermolecular triple helix formation at the target sites A6G6 · C6T6 and G6A6 · T6C6 with the acridine-linked oligonucleotides Acr-T5C5, and Acr-C5T5 respectively. Under pseudo first-order reaction conditions we find that the reactions are slow, with half-lives of several minutes. The rates are dependent on the concentration of the third strand oligonucleotide and yield bimolecular association rate constants of 300 M-1 · s-1 for Acr-T5C5 binding to A6G6 · C6T6 and 2000 M-1 · s-1 for the interaction of Acr-C5T5 with G6A6 · T6C6.

Association kinetics, DNase I footprinting, Triple helix
0014-5793
312-316
Fox, Keith R.
9da5debc-4e45-473e-ab8c-550d1104659f
Fox, Keith R.
9da5debc-4e45-473e-ab8c-550d1104659f

Fox, Keith R. (1995) Kinetic studies on the formation of acridine-linked DNA triple helices. FEBS Letters, 357 (3), 312-316. (doi:10.1016/0014-5793(94)01387-G).

Record type: Article

Abstract

We have used DNase I footprinting to measure the rate of intermolecular triple helix formation at the target sites A6G6 · C6T6 and G6A6 · T6C6 with the acridine-linked oligonucleotides Acr-T5C5, and Acr-C5T5 respectively. Under pseudo first-order reaction conditions we find that the reactions are slow, with half-lives of several minutes. The rates are dependent on the concentration of the third strand oligonucleotide and yield bimolecular association rate constants of 300 M-1 · s-1 for Acr-T5C5 binding to A6G6 · C6T6 and 2000 M-1 · s-1 for the interaction of Acr-C5T5 with G6A6 · T6C6.

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More information

Published date: 9 January 1995
Keywords: Association kinetics, DNase I footprinting, Triple helix
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Identifiers

Local EPrints ID: 475861
URI: http://eprints.soton.ac.uk/id/eprint/475861
DOI: doi:10.1016/0014-5793(94)01387-G
ISSN: 0014-5793
PURE UUID: 763d855d-b57a-45d0-9ed3-90e903ccc199
ORCID for Keith R. Fox: ORCID iD orcid.org/0000-0002-2925-7315

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Date deposited: 29 Mar 2023 16:46
Last modified: 17 Mar 2024 02:34

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Author: Keith R. Fox ORCID iD

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