Nucleosome core particles inhibit DNA triple helix formation
Nucleosome core particles inhibit DNA triple helix formation
We have used DNase I footprinting to examine the formation of DNA triple helices at target sites on DNA fragments that have been reconstituted with nucleosome core particles. We show that a 12 bp homopurine target site, located 45 bp from the end of the 160 bp tyrT(46A) fragment, cannot be targeted with either parallel (CT-containing) or antiparallel (GT-containing) triplex-forming oligonucleotides when reconstituted on to nucleosome core particles. Binding is not facilitated by the presence of a triplex-binding ligand. However, both parallel and antiparallel triplexes could be formed on a truncated DNA fragment in which the target site was located closer to the end of the DNA fragment. We suggest that intermolecular DNA triplexes can only be formed on those DNA regions that are less tightly associated with the protein core.
607-611
Brown, Philip M.
c910b8df-2849-4b26-8f69-3ca330836e9b
Fox, Keith R.
9da5debc-4e45-473e-ab8c-550d1104659f
15 October 1996
Brown, Philip M.
c910b8df-2849-4b26-8f69-3ca330836e9b
Fox, Keith R.
9da5debc-4e45-473e-ab8c-550d1104659f
Brown, Philip M. and Fox, Keith R.
(1996)
Nucleosome core particles inhibit DNA triple helix formation.
Biochemical Journal, 319 (2), .
(doi:10.1042/bj3190607).
Abstract
We have used DNase I footprinting to examine the formation of DNA triple helices at target sites on DNA fragments that have been reconstituted with nucleosome core particles. We show that a 12 bp homopurine target site, located 45 bp from the end of the 160 bp tyrT(46A) fragment, cannot be targeted with either parallel (CT-containing) or antiparallel (GT-containing) triplex-forming oligonucleotides when reconstituted on to nucleosome core particles. Binding is not facilitated by the presence of a triplex-binding ligand. However, both parallel and antiparallel triplexes could be formed on a truncated DNA fragment in which the target site was located closer to the end of the DNA fragment. We suggest that intermolecular DNA triplexes can only be formed on those DNA regions that are less tightly associated with the protein core.
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Published date: 15 October 1996
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Local EPrints ID: 475868
URI: http://eprints.soton.ac.uk/id/eprint/475868
ISSN: 0264-6021
PURE UUID: 9a18d5cd-d68f-4a3f-a47c-fcf175f43406
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Date deposited: 29 Mar 2023 16:46
Last modified: 17 Mar 2024 02:34
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Author:
Philip M. Brown
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