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Clonal expansion of community-associated meticillin-resistant Staphylococcus aureus (MRSA) in people who inject drugs (PWID): prevalence, risk factors and molecular epidemiology, Bristol, United Kingdom, 2012 to 2017

Clonal expansion of community-associated meticillin-resistant Staphylococcus aureus (MRSA) in people who inject drugs (PWID): prevalence, risk factors and molecular epidemiology, Bristol, United Kingdom, 2012 to 2017
Clonal expansion of community-associated meticillin-resistant Staphylococcus aureus (MRSA) in people who inject drugs (PWID): prevalence, risk factors and molecular epidemiology, Bristol, United Kingdom, 2012 to 2017

Background: in 2015, Bristol (South West England) experienced a large increase in cases of meticillin-resistant Staphylococcus aureus (MRSA) infection in people who inject drugs (PWID).

Aim: we aimed to characterise and estimate the prevalence of MRSA colonisation among PWID in Bristol and test evidence of a clonal outbreak.

Methods: PWID recruited through an unlinked-anonymous community survey during 2016 completed behavioural questionnaires and were screened for MRSA. Univariable logistic regression examined associations with MRSA colonisation. Whole-genome sequencing used lineage-matched MRSA isolates, comparing PWID (screening and retrospective bacteraemia samples from 2012-2017) with non-PWID (Bristol screening) in Bristol and national reference laboratory database samples.

Results: the MRSA colonisation prevalence was 8.7% (13/149) and was associated with frequently injecting in public places (odds ratio (OR): 5.5; 95% confidence interval (CI):1.34-22.70), recent healthcare contact (OR: 4.3; 95% CI: 1.34-13.80) and injecting in groups of three or more (OR: 15.8; 95% CI: 2.51-99.28). People reporting any one of: injecting in public places, injection site skin and soft tissue infection or hospital contact accounted for 12/13 MRSA positive cases (sensitivity 92.3%; specificity 51.5%). Phylogenetic analysis identified a dominant clade associated with infection and colonisation among PWID in Bristol belonging to ST5-SCCmecIVg.

Conclusions: MRSA colonisation in Bristol PWID is substantially elevated compared with general population estimates and there is evidence of clonal expansion, community-based transmission and increased infection risk related to the colonising strain. Targeted interventions, including community screening and suppression therapy, education and basic infection control are needed to reduce MRSA infections in PWID.

Adult, Bacteremia/epidemiology, Community-Acquired Infections/epidemiology, Cross-Sectional Studies, Female, Humans, Male, Methicillin-Resistant Staphylococcus aureus/drug effects, Molecular Epidemiology, Molecular Typing, Phylogeny, Prevalence, Retrospective Studies, Risk Factors, Staphylococcal Infections/epidemiology, Substance Abuse, Intravenous/complications, Surveys and Questionnaires, United Kingdom/epidemiology, Whole Genome Sequencing
Packer, Simon
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Pichon, Bruno
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Thompson, Stephen
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Neale, Jane
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Njoroge, Jacquelyn
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Kwiatkowska, Rachel M.
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Oliver, Isabel
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Telfer, Maggie
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Doumith, Michel
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Buunaaisie, Camillus
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Heinsbroek, Ellen
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Hopewell-Kelly, Noreen
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Desai, Monica
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Hope, Vivian
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Williams, Owen Martin
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Kearns, Angela
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Hickman, Mathew
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Gobin, Maya
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Packer, Simon
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Pichon, Bruno
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Thompson, Stephen
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Neale, Jane
7fa842e9-7b0f-4607-bf0d-dd2ff57d2329
Njoroge, Jacquelyn
cef5fd2e-4136-4b8e-b598-f66e9e9399d9
Kwiatkowska, Rachel M.
c37313d1-f59e-400d-9c45-9577bdf5d2df
Oliver, Isabel
1598f598-b35d-42a4-9695-9b57c5ab15e9
Telfer, Maggie
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Doumith, Michel
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Buunaaisie, Camillus
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Heinsbroek, Ellen
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Hopewell-Kelly, Noreen
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Desai, Monica
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Hope, Vivian
41ec03cc-e079-4ff6-851f-46237715354b
Williams, Owen Martin
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Kearns, Angela
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Hickman, Mathew
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Gobin, Maya
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Packer, Simon, Pichon, Bruno, Thompson, Stephen, Neale, Jane, Njoroge, Jacquelyn, Kwiatkowska, Rachel M., Oliver, Isabel, Telfer, Maggie, Doumith, Michel, Buunaaisie, Camillus, Heinsbroek, Ellen, Hopewell-Kelly, Noreen, Desai, Monica, Hope, Vivian, Williams, Owen Martin, Kearns, Angela, Hickman, Mathew and Gobin, Maya (2019) Clonal expansion of community-associated meticillin-resistant Staphylococcus aureus (MRSA) in people who inject drugs (PWID): prevalence, risk factors and molecular epidemiology, Bristol, United Kingdom, 2012 to 2017. Eurosurveillance, 24 (13). (doi:10.2807/1560-7917.ES.2019.24.13.1800124).

Record type: Article

Abstract

Background: in 2015, Bristol (South West England) experienced a large increase in cases of meticillin-resistant Staphylococcus aureus (MRSA) infection in people who inject drugs (PWID).

Aim: we aimed to characterise and estimate the prevalence of MRSA colonisation among PWID in Bristol and test evidence of a clonal outbreak.

Methods: PWID recruited through an unlinked-anonymous community survey during 2016 completed behavioural questionnaires and were screened for MRSA. Univariable logistic regression examined associations with MRSA colonisation. Whole-genome sequencing used lineage-matched MRSA isolates, comparing PWID (screening and retrospective bacteraemia samples from 2012-2017) with non-PWID (Bristol screening) in Bristol and national reference laboratory database samples.

Results: the MRSA colonisation prevalence was 8.7% (13/149) and was associated with frequently injecting in public places (odds ratio (OR): 5.5; 95% confidence interval (CI):1.34-22.70), recent healthcare contact (OR: 4.3; 95% CI: 1.34-13.80) and injecting in groups of three or more (OR: 15.8; 95% CI: 2.51-99.28). People reporting any one of: injecting in public places, injection site skin and soft tissue infection or hospital contact accounted for 12/13 MRSA positive cases (sensitivity 92.3%; specificity 51.5%). Phylogenetic analysis identified a dominant clade associated with infection and colonisation among PWID in Bristol belonging to ST5-SCCmecIVg.

Conclusions: MRSA colonisation in Bristol PWID is substantially elevated compared with general population estimates and there is evidence of clonal expansion, community-based transmission and increased infection risk related to the colonising strain. Targeted interventions, including community screening and suppression therapy, education and basic infection control are needed to reduce MRSA infections in PWID.

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Published date: 28 March 2019
Keywords: Adult, Bacteremia/epidemiology, Community-Acquired Infections/epidemiology, Cross-Sectional Studies, Female, Humans, Male, Methicillin-Resistant Staphylococcus aureus/drug effects, Molecular Epidemiology, Molecular Typing, Phylogeny, Prevalence, Retrospective Studies, Risk Factors, Staphylococcal Infections/epidemiology, Substance Abuse, Intravenous/complications, Surveys and Questionnaires, United Kingdom/epidemiology, Whole Genome Sequencing

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Local EPrints ID: 475923
URI: http://eprints.soton.ac.uk/id/eprint/475923
PURE UUID: c684f8d5-0c82-4176-ad71-103aa44f3c14

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Date deposited: 31 Mar 2023 16:32
Last modified: 17 Mar 2024 01:27

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Contributors

Author: Simon Packer
Author: Bruno Pichon
Author: Stephen Thompson
Author: Jane Neale
Author: Jacquelyn Njoroge
Author: Rachel M. Kwiatkowska
Author: Isabel Oliver
Author: Maggie Telfer
Author: Michel Doumith
Author: Camillus Buunaaisie
Author: Ellen Heinsbroek
Author: Noreen Hopewell-Kelly
Author: Monica Desai
Author: Vivian Hope
Author: Owen Martin Williams
Author: Angela Kearns
Author: Mathew Hickman
Author: Maya Gobin

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