Bdellovibrio bacteriovorus phosphoglucose isomerase structures reveal novel rigidity in the active site of a selected subset of enzymes upon substrate binding
Bdellovibrio bacteriovorus phosphoglucose isomerase structures reveal novel rigidity in the active site of a selected subset of enzymes upon substrate binding
Glycolysis and gluconeogenesis are central pathways of metabolism across all domains of life. A prominent enzyme in these pathways is phosphoglucose isomerase (PGI), which mediates the interconversion of glucose-6-phosphate and fructose-6-phosphate. The predatory bacterium Bdellovibrio bacteriovorus leads a complex life cycle, switching between intraperiplasmic replicative and extracellular ‘hunter’ attack-phase stages. Passage through this complex life cycle involves different metabolic states. Here we present the unliganded and substrate-bound structures of the B. bacteriovorus PGI, solved to 1.74 Å and 1.67 Å, respectively. These structures reveal that an induced-fit conformational change within the active site is not a prerequisite for the binding of substrates in some PGIs. Crucially, we suggest a phenylalanine residue, conserved across most PGI enzymes but substituted for glycine in B. bacteriovorus and other select organisms, is central to the induced-fit mode of substrate recognition for PGIs. This enzyme also represents the smallest conventional PGI characterized to date and probably represents the minimal requirements for a functional PGI.
Meek, R. W.
5fdcf8d0-6b07-4d63-b719-8302fdd7a056
Cadby, I. T.
a6e10011-3061-456c-8c85-db50573ad97f
Lovering, A. L.
836e794a-429e-4af7-80d0-20d1631355d4
August 2021
Meek, R. W.
5fdcf8d0-6b07-4d63-b719-8302fdd7a056
Cadby, I. T.
a6e10011-3061-456c-8c85-db50573ad97f
Lovering, A. L.
836e794a-429e-4af7-80d0-20d1631355d4
Meek, R. W., Cadby, I. T. and Lovering, A. L.
(2021)
Bdellovibrio bacteriovorus phosphoglucose isomerase structures reveal novel rigidity in the active site of a selected subset of enzymes upon substrate binding.
Open Biology.
(doi:10.1098/rsob.210098).
Abstract
Glycolysis and gluconeogenesis are central pathways of metabolism across all domains of life. A prominent enzyme in these pathways is phosphoglucose isomerase (PGI), which mediates the interconversion of glucose-6-phosphate and fructose-6-phosphate. The predatory bacterium Bdellovibrio bacteriovorus leads a complex life cycle, switching between intraperiplasmic replicative and extracellular ‘hunter’ attack-phase stages. Passage through this complex life cycle involves different metabolic states. Here we present the unliganded and substrate-bound structures of the B. bacteriovorus PGI, solved to 1.74 Å and 1.67 Å, respectively. These structures reveal that an induced-fit conformational change within the active site is not a prerequisite for the binding of substrates in some PGIs. Crucially, we suggest a phenylalanine residue, conserved across most PGI enzymes but substituted for glycine in B. bacteriovorus and other select organisms, is central to the induced-fit mode of substrate recognition for PGIs. This enzyme also represents the smallest conventional PGI characterized to date and probably represents the minimal requirements for a functional PGI.
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rsob.210098
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Published date: August 2021
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Local EPrints ID: 476102
URI: http://eprints.soton.ac.uk/id/eprint/476102
PURE UUID: 348ea62d-e28f-4263-be53-253bf5fd79b9
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Date deposited: 12 Apr 2023 14:09
Last modified: 17 Mar 2024 04:19
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Author:
R. W. Meek
Author:
I. T. Cadby
Author:
A. L. Lovering
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