Kinetic studies on the formation of intermolecular triple helices
Kinetic studies on the formation of intermolecular triple helices
We have used DNase I footprinting to examine the association kinetics of GA-, GT- and CT-containing oligonucleotides with the target sequence (GGA)5GG. (CCT)5CC. These reactions are slow yielding bimolecular association rate constants between 50 and 2000 M-1 s-1. We find that GT-containing oligonucleotides bind much faster than GA- or CT-containing third strands. In each case the observed rate constants are faster at the centre than at the edges of the target site. Although several explanations can be offered for this observation, it is consistent with a model in which triplex formation at this repetitive site is achieved via intermediate complexes in which the third strand is not properly aligned with its target and which subsequently migrate to the correct position.
3269-3274
Paes, Hazel M.
ba0c039a-4a53-4e90-9037-fc4c2e6e0076
Fox, Keith R.
9da5debc-4e45-473e-ab8c-550d1104659f
1997
Paes, Hazel M.
ba0c039a-4a53-4e90-9037-fc4c2e6e0076
Fox, Keith R.
9da5debc-4e45-473e-ab8c-550d1104659f
Paes, Hazel M. and Fox, Keith R.
(1997)
Kinetic studies on the formation of intermolecular triple helices.
Nucleic Acids Research, 25 (16), .
(doi:10.1093/nar/25.16.3269).
Abstract
We have used DNase I footprinting to examine the association kinetics of GA-, GT- and CT-containing oligonucleotides with the target sequence (GGA)5GG. (CCT)5CC. These reactions are slow yielding bimolecular association rate constants between 50 and 2000 M-1 s-1. We find that GT-containing oligonucleotides bind much faster than GA- or CT-containing third strands. In each case the observed rate constants are faster at the centre than at the edges of the target site. Although several explanations can be offered for this observation, it is consistent with a model in which triplex formation at this repetitive site is achieved via intermediate complexes in which the third strand is not properly aligned with its target and which subsequently migrate to the correct position.
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Published date: 1997
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Funding Information:
This work was supported by grants from the Cancer Research Campaign and the Medical Research Council.
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Local EPrints ID: 476345
URI: http://eprints.soton.ac.uk/id/eprint/476345
ISSN: 0305-1048
PURE UUID: 271b5cc0-f6d0-4d2a-a9a1-372185ec7f7c
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Date deposited: 19 Apr 2023 16:46
Last modified: 18 Mar 2024 02:32
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Author:
Hazel M. Paes
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