DNA triple-helix formation on nucleosome-bound poly(dA) · poly(dT) tracts
DNA triple-helix formation on nucleosome-bound poly(dA) · poly(dT) tracts
We have used DNase I and hydroxyl-radical footprinting to examine the formation of intermolecular DNA triple helices on nucleosome-bound DNA fragments containing An·Tn tracts. We found that it is possible to form triplexes on these nucleosome-bound DNAs, but the stability of the complexes depends on the orientation of the A tract with respect to the protein surface. Hydroxyl-radical cleavage of these complexes suggests that the DNA fragments are still associated with the nucleosome. However, the phased cleavage pattern is lost in the vicinity of the triplex, suggesting that the DNA has locally moved away from the protein surface.
259-267
Brown, Philip M.
c910b8df-2849-4b26-8f69-3ca330836e9b
Fox, Keith R.
9da5debc-4e45-473e-ab8c-550d1104659f
15 July 1998
Brown, Philip M.
c910b8df-2849-4b26-8f69-3ca330836e9b
Fox, Keith R.
9da5debc-4e45-473e-ab8c-550d1104659f
Brown, Philip M. and Fox, Keith R.
(1998)
DNA triple-helix formation on nucleosome-bound poly(dA) · poly(dT) tracts.
Biochemical Journal, 333 (2), .
(doi:10.1042/bj3330259).
Abstract
We have used DNase I and hydroxyl-radical footprinting to examine the formation of intermolecular DNA triple helices on nucleosome-bound DNA fragments containing An·Tn tracts. We found that it is possible to form triplexes on these nucleosome-bound DNAs, but the stability of the complexes depends on the orientation of the A tract with respect to the protein surface. Hydroxyl-radical cleavage of these complexes suggests that the DNA fragments are still associated with the nucleosome. However, the phased cleavage pattern is lost in the vicinity of the triplex, suggesting that the DNA has locally moved away from the protein surface.
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Published date: 15 July 1998
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Local EPrints ID: 476348
URI: http://eprints.soton.ac.uk/id/eprint/476348
ISSN: 0264-6021
PURE UUID: af1c6370-3560-4ff0-ba5a-425aa05d4b60
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Date deposited: 19 Apr 2023 16:46
Last modified: 17 Mar 2024 02:34
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Author:
Philip M. Brown
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