DNA sequence recognition by a novel series of minor groove-binding ligands
DNA sequence recognition by a novel series of minor groove-binding ligands
We have used quantitative DNase I and hydroxyl radical footprinting with three DNA fragments to examine the sequence-specific recognition of DNA by five novel minor groove-binding ligands which contain a structural motif in which a para-disubstituted benzene ring is flanked by two meta-disubstituted benzene rings. The compounds are all AT-selective and bind better to (AT)6 than (AT)4 sites. The ligands bind more tightly to AATT and TAAT than TTAA, TATA and ATAT, and avoid sequences containing central TpA steps. Different side groups cause subtle changes to the sequence recognition properties of these ligands.
Footprinting, Minor groove ligand, Sequence recognition
219-230
Fox, Keith R.
9da5debc-4e45-473e-ab8c-550d1104659f
Yan, Yinfa
2b37bc2f-3571-4d35-a72a-d91cd997b20d
Gong, Bing
a45b7741-5265-4adb-921f-36041bc4c473
June 1999
Fox, Keith R.
9da5debc-4e45-473e-ab8c-550d1104659f
Yan, Yinfa
2b37bc2f-3571-4d35-a72a-d91cd997b20d
Gong, Bing
a45b7741-5265-4adb-921f-36041bc4c473
Fox, Keith R., Yan, Yinfa and Gong, Bing
(1999)
DNA sequence recognition by a novel series of minor groove-binding ligands.
Anti-Cancer Drug Design, 14 (3), .
Abstract
We have used quantitative DNase I and hydroxyl radical footprinting with three DNA fragments to examine the sequence-specific recognition of DNA by five novel minor groove-binding ligands which contain a structural motif in which a para-disubstituted benzene ring is flanked by two meta-disubstituted benzene rings. The compounds are all AT-selective and bind better to (AT)6 than (AT)4 sites. The ligands bind more tightly to AATT and TAAT than TTAA, TATA and ATAT, and avoid sequences containing central TpA steps. Different side groups cause subtle changes to the sequence recognition properties of these ligands.
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Published date: June 1999
Keywords:
Footprinting, Minor groove ligand, Sequence recognition
Identifiers
Local EPrints ID: 476350
URI: http://eprints.soton.ac.uk/id/eprint/476350
ISSN: 0266-9536
PURE UUID: ddd5c930-ec9e-4206-8df6-34591e1476d7
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Date deposited: 19 Apr 2023 16:46
Last modified: 06 Jun 2024 01:32
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Contributors
Author:
Yinfa Yan
Author:
Bing Gong
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