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DNA sequence recognition by a novel series of minor groove-binding ligands

DNA sequence recognition by a novel series of minor groove-binding ligands
DNA sequence recognition by a novel series of minor groove-binding ligands

We have used quantitative DNase I and hydroxyl radical footprinting with three DNA fragments to examine the sequence-specific recognition of DNA by five novel minor groove-binding ligands which contain a structural motif in which a para-disubstituted benzene ring is flanked by two meta-disubstituted benzene rings. The compounds are all AT-selective and bind better to (AT)6 than (AT)4 sites. The ligands bind more tightly to AATT and TAAT than TTAA, TATA and ATAT, and avoid sequences containing central TpA steps. Different side groups cause subtle changes to the sequence recognition properties of these ligands.

Footprinting, Minor groove ligand, Sequence recognition
0266-9536
219-230
Fox, Keith R.
9da5debc-4e45-473e-ab8c-550d1104659f
Yan, Yinfa
2b37bc2f-3571-4d35-a72a-d91cd997b20d
Gong, Bing
a45b7741-5265-4adb-921f-36041bc4c473
Fox, Keith R.
9da5debc-4e45-473e-ab8c-550d1104659f
Yan, Yinfa
2b37bc2f-3571-4d35-a72a-d91cd997b20d
Gong, Bing
a45b7741-5265-4adb-921f-36041bc4c473

Fox, Keith R., Yan, Yinfa and Gong, Bing (1999) DNA sequence recognition by a novel series of minor groove-binding ligands. Anti-Cancer Drug Design, 14 (3), 219-230.

Record type: Article

Abstract

We have used quantitative DNase I and hydroxyl radical footprinting with three DNA fragments to examine the sequence-specific recognition of DNA by five novel minor groove-binding ligands which contain a structural motif in which a para-disubstituted benzene ring is flanked by two meta-disubstituted benzene rings. The compounds are all AT-selective and bind better to (AT)6 than (AT)4 sites. The ligands bind more tightly to AATT and TAAT than TTAA, TATA and ATAT, and avoid sequences containing central TpA steps. Different side groups cause subtle changes to the sequence recognition properties of these ligands.

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More information

Published date: June 1999
Keywords: Footprinting, Minor groove ligand, Sequence recognition

Identifiers

Local EPrints ID: 476350
URI: http://eprints.soton.ac.uk/id/eprint/476350
ISSN: 0266-9536
PURE UUID: ddd5c930-ec9e-4206-8df6-34591e1476d7
ORCID for Keith R. Fox: ORCID iD orcid.org/0000-0002-2925-7315

Catalogue record

Date deposited: 19 Apr 2023 16:46
Last modified: 20 Feb 2024 02:32

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Contributors

Author: Keith R. Fox ORCID iD
Author: Yinfa Yan
Author: Bing Gong

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