DNA triple helix stabilisation by covalent attachment of a triplex-specific ligand
DNA triple helix stabilisation by covalent attachment of a triplex-specific ligand
We have prepared oligonucleotides with a naphthylquinoline triplex-binding ligand covalently tethered to the 5'-end and have used UV-melting and DNase I footprinting to examine the stability of intra- and inter-molecular triplexes containing this modification. We find that covalent attachment of the ligand increases the melting temperature of intramolecular 6-mer triplexes by about 14 K, and increases the binding of 9-mer oligonucleotides to their duplex target sites by about 60-fold. Copyright (C) 1999 Elsevier Science B.V.
DNA triplex, Footprinting, Naphthylquinoline, Triplex ligand
137-145
Keppler, Melanie D.
6452c8eb-268a-47f4-955d-e1819df05333
McKeen, Catherine M.
44153a47-668b-4cfa-808c-15552c9f74d8
Zegrocka, Oliwia
da3025f5-05c6-4b8c-92ae-3331eb04d136
Strekowski, Lucjan
a671c874-b36b-41ca-affe-ce373f032209
Brown, Tom
a64aae36-bb30-42df-88a2-11be394e8c89
Fox, Keith R.
9da5debc-4e45-473e-ab8c-550d1104659f
28 October 1999
Keppler, Melanie D.
6452c8eb-268a-47f4-955d-e1819df05333
McKeen, Catherine M.
44153a47-668b-4cfa-808c-15552c9f74d8
Zegrocka, Oliwia
da3025f5-05c6-4b8c-92ae-3331eb04d136
Strekowski, Lucjan
a671c874-b36b-41ca-affe-ce373f032209
Brown, Tom
a64aae36-bb30-42df-88a2-11be394e8c89
Fox, Keith R.
9da5debc-4e45-473e-ab8c-550d1104659f
Keppler, Melanie D., McKeen, Catherine M., Zegrocka, Oliwia, Strekowski, Lucjan, Brown, Tom and Fox, Keith R.
(1999)
DNA triple helix stabilisation by covalent attachment of a triplex-specific ligand.
Biochimica et Biophysica Acta - Gene Structure and Expression, 1447 (2-3), .
(doi:10.1016/S0167-4781(99)00114-1).
Abstract
We have prepared oligonucleotides with a naphthylquinoline triplex-binding ligand covalently tethered to the 5'-end and have used UV-melting and DNase I footprinting to examine the stability of intra- and inter-molecular triplexes containing this modification. We find that covalent attachment of the ligand increases the melting temperature of intramolecular 6-mer triplexes by about 14 K, and increases the binding of 9-mer oligonucleotides to their duplex target sites by about 60-fold. Copyright (C) 1999 Elsevier Science B.V.
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Published date: 28 October 1999
Additional Information:
Funding Information:
This work was supported by grants from the Cancer Research Campaign and the Petroleum Research Fund administered by the American Chemical Society.
Keywords:
DNA triplex, Footprinting, Naphthylquinoline, Triplex ligand
Identifiers
Local EPrints ID: 476353
URI: http://eprints.soton.ac.uk/id/eprint/476353
ISSN: 0167-4781
PURE UUID: 98ae6d1d-be45-4bc2-b9d8-7c094245f0ff
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Date deposited: 19 Apr 2023 16:46
Last modified: 18 Mar 2024 02:32
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Contributors
Author:
Melanie D. Keppler
Author:
Catherine M. McKeen
Author:
Oliwia Zegrocka
Author:
Lucjan Strekowski
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