DNA triple helix stabilisation by a naphthylquinoline dimer

Keppler, Melanie, Zegrocka, Oliwia, Strekowski, Lucjan and Fox, Keith R. (1999) DNA triple helix stabilisation by a naphthylquinoline dimer. FEBS Letters, 447 (2-3), 223-226. (doi:10.1016/S0014-5793(99)00284-7).

Record type: Article

Abstract

We have used DNase I footprinting to examine the effect of a novel naphthylquinoline dimer, designed as a triplex-specific bis-intercalator, on the stability of intermolecular DNA triplexes. We find that this compound efficiently promotes triplex formation between the 9-mer oligonucleotide 5'-TTTTTTCTT and its oligopurine duplex target at concentrations as low as 0.1 μM, enhancing the triplex stability by at least 1000-fold. This compound, which is the first reported example of a triplex bis-intercalator, is about 30 times more potent than the simple monofunctional ligand. Copyright (C) 1999 Federation of European Biochemical Societies.

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Published date: 26 March 1999

Additional Information: Funding Information: This work was supported by grants from the Cancer Research Campaign (K.R.F.) and the Petroleum Research Fund, administered by the American Chemical Society (L.S.).

Keywords: Bis-intercalator, DNA triplex, Footprinting, Naphthylquinoline, Triplex ligand

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