Operando NMR metabolomics of a microfluidic cell culture
Operando NMR metabolomics of a microfluidic cell culture
In this work we demonstrate the use of microfluidic NMR for in situ culture and quantitative analysis of metabolism in hepatocellular carcinoma (HCC) cell lines. A hydrothermal heating system is used to enable continuous in situ NMR observation of HCC cell culture over a 24 h incubation period. This technique is nondestructive, non-invasive and can measure millimolar concentrations at microlitre volumes, within a few minutes and in precisely controlled culture conditions. This is sufficient to observe changes in primary energy metabolism, using around 500–3500 cells per device, and with a time resolution of 17 min. The ability to observe intracellular responses in a time-resolved manner provides a more detailed view of a biological system and how it reacts to stimuli. This capability will allow detailed metabolomic studies of cell-culture based cancer models, enabling quantification of metabolic reporgramming, the metabolic tumor microenvironment, and the metabolic interplay between cancer- and immune cells.
Rogers, Genevieve Alice
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Ostrowska, Sylwia
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Sharma, Manvendra
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Khakoo, Salim
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Utz, Marcel
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24 February 2023
Rogers, Genevieve Alice
95d86839-eb6a-4318-bc46-e8920e34af1c
Ostrowska, Sylwia
e43994fe-2f73-41c8-b197-f56a8e00ea5e
Sharma, Manvendra
e249236d-221d-4e59-8440-011f6863f891
Khakoo, Salim
6c16d2f5-ae80-4d9b-9100-6bfb34ad0273
Utz, Marcel
c84ed64c-9e89-4051-af39-d401e423891b
Rogers, Genevieve Alice, Ostrowska, Sylwia, Sharma, Manvendra, Khakoo, Salim and Utz, Marcel
(2023)
Operando NMR metabolomics of a microfluidic cell culture.
Journal of Magnetic Resonance, 349, [107405].
(doi:10.1016/j.jmr.2023.107405).
Abstract
In this work we demonstrate the use of microfluidic NMR for in situ culture and quantitative analysis of metabolism in hepatocellular carcinoma (HCC) cell lines. A hydrothermal heating system is used to enable continuous in situ NMR observation of HCC cell culture over a 24 h incubation period. This technique is nondestructive, non-invasive and can measure millimolar concentrations at microlitre volumes, within a few minutes and in precisely controlled culture conditions. This is sufficient to observe changes in primary energy metabolism, using around 500–3500 cells per device, and with a time resolution of 17 min. The ability to observe intracellular responses in a time-resolved manner provides a more detailed view of a biological system and how it reacts to stimuli. This capability will allow detailed metabolomic studies of cell-culture based cancer models, enabling quantification of metabolic reporgramming, the metabolic tumor microenvironment, and the metabolic interplay between cancer- and immune cells.
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Accepted/In Press date: 12 February 2023
e-pub ahead of print date: 21 February 2023
Published date: 24 February 2023
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Funding Information:
This work has been supported by the ’HUNTER’ accelerator award from CRUK and the Institute for Life Sciences as part of the University of Southampton. This project has also received funding from EPSRC Grant No. EP/W020343/1.
Publisher Copyright:
© 2023 The Author(s)
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Local EPrints ID: 476633
URI: http://eprints.soton.ac.uk/id/eprint/476633
ISSN: 1090-7807
PURE UUID: c86a495b-a254-456f-b057-f1adf0ac7a54
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Date deposited: 10 May 2023 16:43
Last modified: 04 May 2024 02:03
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Author:
Genevieve Alice Rogers
Author:
Sylwia Ostrowska
Author:
Manvendra Sharma
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