Droplet fluidics for time-dependent analysis of barrier permeability in an epithelial barrier on chip system
Droplet fluidics for time-dependent analysis of barrier permeability in an epithelial barrier on chip system
A droplet generator has been developed that interfaces with a barrier-on-chip platform for temporal analyte compartmentalisation and analysis. Droplets are generated every 20 minutes in 8 separate parallel microchannels, with an average droplet volume of 9.47 ± 0.6 μL, allowing simultaneous analysis of 8 different experiments. The device was tested using an epithelial barrier model by monitoring the diffusion of a fluorescent high molecular weight dextran molecule. The epithelial barrier was perturbed using detergent leading to a peak at 3–4 hours, correlating with simulations. For the untreated (control) a constant, very low level of dextran diffusion was observed. The epithelial cell barrier properties were also continuously measured using electrical impedance spectroscopy to extract an equivalent trans epithelial resistance.
14494-14500
Fernandes, Joao C
2dda889e-de40-44f7-bed8-8e40ab79f610
Karra, Nikita
8037beb7-98d3-40b2-9e02-51e4e917b3c0
Swindle, Emily
fe393c7a-a513-4de4-b02e-27369bd7e84f
Morgan, Hywel
de00d59f-a5a2-48c4-a99a-1d5dd7854174
11 May 2023
Fernandes, Joao C
2dda889e-de40-44f7-bed8-8e40ab79f610
Karra, Nikita
8037beb7-98d3-40b2-9e02-51e4e917b3c0
Swindle, Emily
fe393c7a-a513-4de4-b02e-27369bd7e84f
Morgan, Hywel
de00d59f-a5a2-48c4-a99a-1d5dd7854174
Fernandes, Joao C, Karra, Nikita, Swindle, Emily and Morgan, Hywel
(2023)
Droplet fluidics for time-dependent analysis of barrier permeability in an epithelial barrier on chip system.
RSC Advances, 13 (21), , [14494].
(doi:10.1039/D3RA00470H).
Abstract
A droplet generator has been developed that interfaces with a barrier-on-chip platform for temporal analyte compartmentalisation and analysis. Droplets are generated every 20 minutes in 8 separate parallel microchannels, with an average droplet volume of 9.47 ± 0.6 μL, allowing simultaneous analysis of 8 different experiments. The device was tested using an epithelial barrier model by monitoring the diffusion of a fluorescent high molecular weight dextran molecule. The epithelial barrier was perturbed using detergent leading to a peak at 3–4 hours, correlating with simulations. For the untreated (control) a constant, very low level of dextran diffusion was observed. The epithelial cell barrier properties were also continuously measured using electrical impedance spectroscopy to extract an equivalent trans epithelial resistance.
Text
Droplet Fluidics for Time-dependent Analysis of Barrier Permeability in an Epithelial Barrier on Chip Systems
- Accepted Manuscript
Text
d3ra00470h
- Version of Record
More information
Accepted/In Press date: 3 May 2023
e-pub ahead of print date: 11 May 2023
Published date: 11 May 2023
Additional Information:
Funding Information:
This work was funded by the EPSRC and GlaxoSmithKline. We would like to thank Katie Chamberlain for her assistance with fabrication of glass chips and Mark Long, Anthony Gardener and Jamie Stone for their technical assistance with micromilling and 3D printing. The 16HBE14o-cell line was gifted from Prof. D. C. Gruenert, San Francisco, USA.
Publisher Copyright:
© 2023 The Royal Society of Chemistry
Identifiers
Local EPrints ID: 476922
URI: http://eprints.soton.ac.uk/id/eprint/476922
ISSN: 2046-2069
PURE UUID: 2cc2b875-ce44-4889-87f9-efb17fe9cfb0
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Date deposited: 19 May 2023 16:38
Last modified: 06 Jun 2024 01:47
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Author:
Joao C Fernandes
Author:
Nikita Karra
Author:
Hywel Morgan
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