Droplet fluidics for time-dependent analysis of barrier permeability in an epithelial barrier on chip systems

Fernandes, Joao C, Karra, Nikita, Swindle, Emily and Morgan, Hywel (2023) Droplet fluidics for time-dependent analysis of barrier permeability in an epithelial barrier on chip systems. RSC Advances, 13 (21), 14494-14500, [14494]. (doi:10.1039/D3RA00470H).

Record type: Article

Abstract

A droplet generator has been developed that interfaces with a barrier-on-chip platform for temporal analyte compartmentalisation and analysis. Droplets are generated every 20 minutes in 8 separate parallel microchannels, with an average droplet volume of 9.47 ± 0.6 μL, allowing simultaneous analysis of 8 different experiments. The device was tested using an epithelial barrier model by monitoring the diffusion of a fluorescent high molecular weight dextran molecule. The epithelial barrier was perturbed using detergent leading to a peak at 3–4 hours, correlating with simulations. For the untreated (control) a constant, very low level of dextran diffusion was observed. The epithelial cell barrier properties were also continuously measured using electrical impedance spectroscopy to extract an equivalent trans epithelial resistance.

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Droplet Fluidics for Time-dependent Analysis of Barrier Permeability in an Epithelial Barrier on Chip Systems - Accepted Manuscript

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d3ra00470h - Version of Record

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