READ ME File For 'DROPLET FLUIDICS FOR TIME-DEPENDENT ANALYSIS OF BARRIER PERMEABILITY IN AN EPITHELIAL BARRIER ON CHIP SYSTEM'

Dataset DOI: https://doi.org/10.5258/SOTON/D2378

Date that the file was created: 09/2022

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GENERAL INFORMATION
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ReadMe Author: Joao Fernandes, University of Southampton [0000-0002-4504-8249]

Date of data collection: Data was collected in 2020 and 2021.

Information about geographic location of data collection: University of Southampton, Southampton, United Kingdom

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SHARING/ACCESS INFORMATION
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Licenses/restrictions placed on the data, or limitations of reuse: CC-BY

This dataset supports the publication:
AUTHORS:João Fernandes, Nikita Karra, Emily J. Swindle, Hywel Morgan
TITLE: DROPLET FLUIDICS FOR TIME-DEPENDENT ANALYSIS OF BARRIER PERMEABILITY IN AN EPITHELIAL BARRIER ON CHIP SYSTEM
JOURNAL: RSC Advances
https://doi.org/10.1039/D3RA00470H
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DATA & FILE OVERVIEW
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This dataset contains:

	. CellData.zip - Zipped folder with data collected for 16HBE14o- experiments with Triton and dextran-FITC. Data includes both fluorescence and impedance spectroscopy.
	. DropletData.opju - Project folder to be opened using Origin 2021 or latest. Collection of all data used in the paper, including COMSOL simulations, droplet size variation and cell data.
	. FITC_8_Channel_Device.xlsx - Excel file with data collected during FITC apical-to-basolateral diffusion throug the microfluidic chip. To be opened with Excel compatible software.
	. DropletSizeVariation.xlsx - Excel file with data collected for droplet size generation used to assess droplet size variation over 33 hours. To be opened with Excel compatible software.  

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METHODOLOGICAL INFORMATION
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Methods for processing the data: Submitted data was generated from impedance spectroscopy spectras. These spectras were fitted to a defined electrical circuit model, which is detailed in the publication. 

People involved with sample collection, processing, analysis and/or submission: Joao Fernandes, Nikita Karra