Controlling the structure of supramolecular fibre formation for benzothiazole based hydrogels with antimicrobial activity against methicillin resistant staphylococcus aureus
Controlling the structure of supramolecular fibre formation for benzothiazole based hydrogels with antimicrobial activity against methicillin resistant staphylococcus aureus
Antimicrobial resistance is one of the greatest threats to human health. Gram-positive methicillin resistant Staphylococcus aureus (MRSA), in both its planktonic and biofilm form, is of particular concern. Herein we identify the hydrogelation properties for a series of intrinsically fluorescent, structurally related supramolecular self-associating amphiphiles and determine their efficacy against both planktonic and biofilm forms of MRSA. To further explore the potential translation of this hydrogel technology for real-world applications, the toxicity of the amphiphiles was determined against the eukaryotic multicellular model organism, Caenorhabditis elegans. Due to the intrinsic fluorescent nature of these supramolecular amphiphiles, material characterisation of their molecular self-associating properties included; comparative optical density plate reader assays, rheometry and widefield fluorescence microscopy. This enabled determination of amphiphile structure and hydrogel sol dependence on resultant fibre formation.
3958-3968
Hilton, Kira L.F.
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Karamalegkos, Antonis A.
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Allen, Nyasha
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Gwynne, Lauren
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Streather, Bree
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White, Lisa J.
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Baker, Karen B.
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Henry, Samantha A.
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Williams, George T.
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Shepherd, Helena J.
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Shepherd, Mark
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Hind, Charlotte K.
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Sutton, Mark J.
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Jenkins, Toby A.
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Mulvihill, Daniel P.
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Tullet, Jennifer M.A.
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Ezcurra, Marina
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Hiscock, Jennifer R.
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12 April 2023
Hilton, Kira L.F.
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Karamalegkos, Antonis A.
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Allen, Nyasha
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Gwynne, Lauren
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Streather, Bree
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White, Lisa J.
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Baker, Karen B.
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Henry, Samantha A.
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Williams, George T.
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Shepherd, Helena J.
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Shepherd, Mark
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Hind, Charlotte K.
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Sutton, Mark J.
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Jenkins, Toby A.
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Mulvihill, Daniel P.
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Tullet, Jennifer M.A.
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Ezcurra, Marina
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Hiscock, Jennifer R.
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Hilton, Kira L.F., Karamalegkos, Antonis A., Allen, Nyasha, Gwynne, Lauren, Streather, Bree, White, Lisa J., Baker, Karen B., Henry, Samantha A., Williams, George T., Shepherd, Helena J., Shepherd, Mark, Hind, Charlotte K., Sutton, Mark J., Jenkins, Toby A., Mulvihill, Daniel P., Tullet, Jennifer M.A., Ezcurra, Marina and Hiscock, Jennifer R.
(2023)
Controlling the structure of supramolecular fibre formation for benzothiazole based hydrogels with antimicrobial activity against methicillin resistant staphylococcus aureus.
Journal of Materials Chemistry B, 11 (17), .
(doi:10.1039/d3tb00461a).
Abstract
Antimicrobial resistance is one of the greatest threats to human health. Gram-positive methicillin resistant Staphylococcus aureus (MRSA), in both its planktonic and biofilm form, is of particular concern. Herein we identify the hydrogelation properties for a series of intrinsically fluorescent, structurally related supramolecular self-associating amphiphiles and determine their efficacy against both planktonic and biofilm forms of MRSA. To further explore the potential translation of this hydrogel technology for real-world applications, the toxicity of the amphiphiles was determined against the eukaryotic multicellular model organism, Caenorhabditis elegans. Due to the intrinsic fluorescent nature of these supramolecular amphiphiles, material characterisation of their molecular self-associating properties included; comparative optical density plate reader assays, rheometry and widefield fluorescence microscopy. This enabled determination of amphiphile structure and hydrogel sol dependence on resultant fibre formation.
Text
d3tb00461a
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Accepted/In Press date: 11 April 2023
Published date: 12 April 2023
Additional Information:
Funding Information:
KLFH, LJW and JRH would like to thank the University of Kent for funding. AAK would like to thank the GCDC for funding. KLFH and JRH would like to thank Dr John Stolz for his help reviewing this document. LJW and JRH would also like to thank the UKRI (MR/T020415/1) for funding.
Identifiers
Local EPrints ID: 477339
URI: http://eprints.soton.ac.uk/id/eprint/477339
ISSN: 2050-750X
PURE UUID: 9fc6bf51-2662-4261-a0b8-c9785b41f4a6
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Date deposited: 05 Jun 2023 16:37
Last modified: 28 Aug 2024 02:12
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Contributors
Author:
Kira L.F. Hilton
Author:
Antonis A. Karamalegkos
Author:
Nyasha Allen
Author:
Lauren Gwynne
Author:
Bree Streather
Author:
Lisa J. White
Author:
Karen B. Baker
Author:
Samantha A. Henry
Author:
George T. Williams
Author:
Helena J. Shepherd
Author:
Mark Shepherd
Author:
Charlotte K. Hind
Author:
Mark J. Sutton
Author:
Toby A. Jenkins
Author:
Daniel P. Mulvihill
Author:
Jennifer M.A. Tullet
Author:
Marina Ezcurra
Author:
Jennifer R. Hiscock
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