Controlling the structure of supramolecular fibre formation for benzothiazole based hydrogels with antimicrobial activity against methicillin resistant staphylococcus aureus
Controlling the structure of supramolecular fibre formation for benzothiazole based hydrogels with antimicrobial activity against methicillin resistant staphylococcus aureus
Antimicrobial resistance is one of the greatest threats to human health. Gram-positive methicillin resistant Staphylococcus aureus (MRSA), in both its planktonic and biofilm form, is of particular concern. Herein we identify the hydrogelation properties for a series of intrinsically fluorescent, structurally related supramolecular self-associating amphiphiles and determine their efficacy against both planktonic and biofilm forms of MRSA. To further explore the potential translation of this hydrogel technology for real-world applications, the toxicity of the amphiphiles was determined against the eukaryotic multicellular model organism, Caenorhabditis elegans. Due to the intrinsic fluorescent nature of these supramolecular amphiphiles, material characterisation of their molecular self-associating properties included; comparative optical density plate reader assays, rheometry and widefield fluorescence microscopy. This enabled determination of amphiphile structure and hydrogel sol dependence on resultant fibre formation.
3958-3968
Hilton, Kira L.F.
cc0687ab-e382-4117-9407-ab836b6f94e3
Karamalegkos, Antonis A.
0c917125-62f0-4c3f-aefb-633d19443a4f
Allen, Nyasha
340bcea8-ba6f-4500-b739-4d41b25bdcca
Gwynne, Lauren
98d6197d-32de-42a3-bd48-7e21e1b877f2
Streather, Bree
ca2999b3-c0fb-4375-890e-ae6a15d6530a
White, Lisa J.
0ceba55d-c321-4d47-bc70-9f095dc7d9ff
Baker, Karen B.
8ca15089-cc10-4778-ac05-29807448e789
Henry, Samantha A.
6c3f8325-a040-4e10-ae71-f93ac1a3b062
Williams, George T.
26810522-92ef-4b61-a766-582bf15be280
Shepherd, Helena J.
70664bbb-30b9-4f37-bc31-a8a0e2dc8894
Shepherd, Mark
73542f54-b267-4814-9250-9c8b4e2bb078
Hind, Charlotte K.
fffbf552-21d9-41dd-9b0e-f75d71e2ab4e
Sutton, Mark J.
b6a22e5d-9ba1-454f-8153-8c5e26346300
Jenkins, Toby A.
e0e4ebe3-c2d6-4aae-81eb-66923f6f98dd
Mulvihill, Daniel P.
702f83c0-c2e5-48f0-b745-62a7976a248d
Tullet, Jennifer M.A.
bf834087-ea85-4840-8f3f-ced76bcfb6b2
Ezcurra, Marina
4d1b1608-0358-44ee-86a9-55ce0019051d
Hiscock, Jennifer R.
2e1470fc-e298-492e-b50a-f1b590d224db
12 April 2023
Hilton, Kira L.F.
cc0687ab-e382-4117-9407-ab836b6f94e3
Karamalegkos, Antonis A.
0c917125-62f0-4c3f-aefb-633d19443a4f
Allen, Nyasha
340bcea8-ba6f-4500-b739-4d41b25bdcca
Gwynne, Lauren
98d6197d-32de-42a3-bd48-7e21e1b877f2
Streather, Bree
ca2999b3-c0fb-4375-890e-ae6a15d6530a
White, Lisa J.
0ceba55d-c321-4d47-bc70-9f095dc7d9ff
Baker, Karen B.
8ca15089-cc10-4778-ac05-29807448e789
Henry, Samantha A.
6c3f8325-a040-4e10-ae71-f93ac1a3b062
Williams, George T.
26810522-92ef-4b61-a766-582bf15be280
Shepherd, Helena J.
70664bbb-30b9-4f37-bc31-a8a0e2dc8894
Shepherd, Mark
73542f54-b267-4814-9250-9c8b4e2bb078
Hind, Charlotte K.
fffbf552-21d9-41dd-9b0e-f75d71e2ab4e
Sutton, Mark J.
b6a22e5d-9ba1-454f-8153-8c5e26346300
Jenkins, Toby A.
e0e4ebe3-c2d6-4aae-81eb-66923f6f98dd
Mulvihill, Daniel P.
702f83c0-c2e5-48f0-b745-62a7976a248d
Tullet, Jennifer M.A.
bf834087-ea85-4840-8f3f-ced76bcfb6b2
Ezcurra, Marina
4d1b1608-0358-44ee-86a9-55ce0019051d
Hiscock, Jennifer R.
2e1470fc-e298-492e-b50a-f1b590d224db
Hilton, Kira L.F., Karamalegkos, Antonis A., Allen, Nyasha, Gwynne, Lauren, Streather, Bree, White, Lisa J., Baker, Karen B., Henry, Samantha A., Williams, George T., Shepherd, Helena J., Shepherd, Mark, Hind, Charlotte K., Sutton, Mark J., Jenkins, Toby A., Mulvihill, Daniel P., Tullet, Jennifer M.A., Ezcurra, Marina and Hiscock, Jennifer R.
(2023)
Controlling the structure of supramolecular fibre formation for benzothiazole based hydrogels with antimicrobial activity against methicillin resistant staphylococcus aureus.
Journal of Materials Chemistry B, 11 (17), .
(doi:10.1039/d3tb00461a).
Abstract
Antimicrobial resistance is one of the greatest threats to human health. Gram-positive methicillin resistant Staphylococcus aureus (MRSA), in both its planktonic and biofilm form, is of particular concern. Herein we identify the hydrogelation properties for a series of intrinsically fluorescent, structurally related supramolecular self-associating amphiphiles and determine their efficacy against both planktonic and biofilm forms of MRSA. To further explore the potential translation of this hydrogel technology for real-world applications, the toxicity of the amphiphiles was determined against the eukaryotic multicellular model organism, Caenorhabditis elegans. Due to the intrinsic fluorescent nature of these supramolecular amphiphiles, material characterisation of their molecular self-associating properties included; comparative optical density plate reader assays, rheometry and widefield fluorescence microscopy. This enabled determination of amphiphile structure and hydrogel sol dependence on resultant fibre formation.
Text
d3tb00461a
- Version of Record
More information
Accepted/In Press date: 11 April 2023
Published date: 12 April 2023
Additional Information:
Funding Information:
KLFH, LJW and JRH would like to thank the University of Kent for funding. AAK would like to thank the GCDC for funding. KLFH and JRH would like to thank Dr John Stolz for his help reviewing this document. LJW and JRH would also like to thank the UKRI (MR/T020415/1) for funding.
Identifiers
Local EPrints ID: 477339
URI: http://eprints.soton.ac.uk/id/eprint/477339
ISSN: 2050-750X
PURE UUID: 9fc6bf51-2662-4261-a0b8-c9785b41f4a6
Catalogue record
Date deposited: 05 Jun 2023 16:37
Last modified: 18 Mar 2024 04:09
Export record
Altmetrics
Contributors
Author:
Kira L.F. Hilton
Author:
Antonis A. Karamalegkos
Author:
Nyasha Allen
Author:
Lauren Gwynne
Author:
Bree Streather
Author:
Lisa J. White
Author:
Karen B. Baker
Author:
Samantha A. Henry
Author:
George T. Williams
Author:
Helena J. Shepherd
Author:
Mark Shepherd
Author:
Charlotte K. Hind
Author:
Mark J. Sutton
Author:
Toby A. Jenkins
Author:
Daniel P. Mulvihill
Author:
Jennifer M.A. Tullet
Author:
Marina Ezcurra
Author:
Jennifer R. Hiscock
Download statistics
Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.
View more statistics