Investigation of Upper Respiratory Carriage of Bacterial Pathogens among University Students in Kampar, Malaysia
Investigation of Upper Respiratory Carriage of Bacterial Pathogens among University Students in Kampar, Malaysia
The carriage of bacterial pathogens in the human upper respiratory tract (URT) is associated with a risk of invasive respiratory tract infections, but the related epidemiological information on this at the population level is scarce in Malaysia. This study aimed to investigate the URT carriage of Streptococcus pneumoniae, Haemophilus influenzae, Neisseria meningitidis, Staphylococcus aureus, Klebsiella pneumoniae and Pseudomonas aeruginosa among 100 university students by nasal and oropharyngeal swabbing. The presence of S. aureus, K. pneumoniae and P. aeruginosa was assessed via swab culture on selective media and PCR on the resulting isolates. For S. pneumoniae, H. influenzae and N. meningitidis, their presence was assessed via multiplex PCR on the total DNA extracts from chocolate agar cultures. The carriage prevalence of H. influenzae, S. aureus, S. pneumoniae, K. pneumoniae, N. meningitidis and P. aeruginosa among the subjects was 36%, 27%, 15%, 11%, 5% and 1%, respectively, by these approaches. Their carriage was significantly higher in males compared to females overall. The S. aureus, K. pneumoniae and P. aeruginosa isolates were also screened by the Kirby-Bauer assay, in which 51.6% of S. aureus were penicillin-resistant. The outcomes from carriage studies are expected to contribute to informing infectious disease control policies and guidelines.
carriage study, Haemophilus influenzae, Klebsiella pneumoniae, Malaysia, Neisseria meningitidis, Pseudomonas aeruginosa, Staphylococcus aureus, Streptococcus pneumoniae, upper respiratory tract
Huat Ong, Hing
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Keat Toh, Wai
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Ying Thong, Li
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Quen Phoon, Lee
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Clarke, Stuart C.
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Cheah, Eddy Seong Guan
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8 May 2023
Huat Ong, Hing
28edeeb7-37fd-46d8-a7b7-349123901a19
Keat Toh, Wai
e9e33d36-f7ae-4d67-b953-cd189714471f
Ying Thong, Li
f3369a3d-cf60-4d71-86c1-d733d92e888a
Quen Phoon, Lee
44ad8b2b-48d8-443c-81b2-8ef71d4d6280
Clarke, Stuart C.
f7d7f7a2-4b1f-4b36-883a-0f967e73fb17
Cheah, Eddy Seong Guan
b409b18b-d6b8-424e-be35-7ffb35a7f857
Huat Ong, Hing, Keat Toh, Wai, Ying Thong, Li, Quen Phoon, Lee, Clarke, Stuart C. and Cheah, Eddy Seong Guan
(2023)
Investigation of Upper Respiratory Carriage of Bacterial Pathogens among University Students in Kampar, Malaysia.
Tropical Medicine and Infectious Disease, 8 (5), [269].
(doi:10.3390/tropicalmed8050269).
Abstract
The carriage of bacterial pathogens in the human upper respiratory tract (URT) is associated with a risk of invasive respiratory tract infections, but the related epidemiological information on this at the population level is scarce in Malaysia. This study aimed to investigate the URT carriage of Streptococcus pneumoniae, Haemophilus influenzae, Neisseria meningitidis, Staphylococcus aureus, Klebsiella pneumoniae and Pseudomonas aeruginosa among 100 university students by nasal and oropharyngeal swabbing. The presence of S. aureus, K. pneumoniae and P. aeruginosa was assessed via swab culture on selective media and PCR on the resulting isolates. For S. pneumoniae, H. influenzae and N. meningitidis, their presence was assessed via multiplex PCR on the total DNA extracts from chocolate agar cultures. The carriage prevalence of H. influenzae, S. aureus, S. pneumoniae, K. pneumoniae, N. meningitidis and P. aeruginosa among the subjects was 36%, 27%, 15%, 11%, 5% and 1%, respectively, by these approaches. Their carriage was significantly higher in males compared to females overall. The S. aureus, K. pneumoniae and P. aeruginosa isolates were also screened by the Kirby-Bauer assay, in which 51.6% of S. aureus were penicillin-resistant. The outcomes from carriage studies are expected to contribute to informing infectious disease control policies and guidelines.
Text
tropicalmed-08-00269-v2
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More information
Accepted/In Press date: 2 May 2023
e-pub ahead of print date: 8 May 2023
Published date: 8 May 2023
Additional Information:
Funding Information:
The research was supported by the Ministry of Higher Education (MoHE), through the Fundamental Research Grant Scheme (FRGS/1/2018/SKK06/UTAR/02/4), and the Universiti Tunku Abdul Rahman (UTAR) Research Fund (IPSR/RMC/UTARRF/2017-C2/E01).
Publisher Copyright:
© 2023 by the authors.
Keywords:
carriage study, Haemophilus influenzae, Klebsiella pneumoniae, Malaysia, Neisseria meningitidis, Pseudomonas aeruginosa, Staphylococcus aureus, Streptococcus pneumoniae, upper respiratory tract
Identifiers
Local EPrints ID: 477360
URI: http://eprints.soton.ac.uk/id/eprint/477360
PURE UUID: 5f70ae08-7818-4dc5-a7b5-31a627ba9350
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Date deposited: 05 Jun 2023 16:43
Last modified: 17 Mar 2024 03:07
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Contributors
Author:
Hing Huat Ong
Author:
Wai Keat Toh
Author:
Li Ying Thong
Author:
Lee Quen Phoon
Author:
Eddy Seong Guan Cheah
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