READ ME file for 'Dataset for Marine Biofilm Viscoelasticity"

Dataset DOI: https://doi.org/10.5258/SOTON/D2439

File was created: November 2022

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GENERAL INFORMATION
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ReadMe Author: Alexandra Snowdon (formerly Jackson), University of Southampton [ORCID ID, 0000-0001-6672-937X]
Date of data collection: February - July 2022

Information about geographic location of data collection: 
Data was collected and processed at two sites: Newcastle University Dove Laboratory (Cullercoats, UK) and AkzoNobel (Gateshead, UK)
1) Dove Laboratory
	Marine Biofilms grown in a natural seawater tank, 7 - 10oC
	
2) AkzoNobel
	Preparing and painting coupons
	Optical Coherence Tomogrpaphy (OCT) - structural data of marine biofilms
	Parallel-plate Rheometer - rheological characterisation of marine biofilms

This work was funded by a DTP ESPRC grant EP/R513325/1 to the University of Southampton with partial funding AkzoNobel. 

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SHARING/ACCESS INFORMATION
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Licenses/restrictions placed on the data, or limitations of reuse:CC-BY

Recommended citation for the data:

This dataset supports the publication:
AUTHORS: Alexandra Snowdon, Simon Dennington, Jennifer Longyear, Julian Wharton, Paul Stoodley
TITLE: 
JOURNAL: Soft Matter
PAPER DOI IF KNOWN: https://doi.org/10.1039/D2SM01647H


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DATA & FILE OVERVIEW 
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This dataset contains:

Each file refers to sructural and mechanical data collected for three different surface types:
	FRC - a foul release coating (AkzoNobel)
	ACP - an anti-corrosive prime with no antifouling properties (AkzoNobel)
	PVC - plain sanded PVC coupons 

Each excel file has a README tab incorporated but briefly: 
1) Coupon_SurfaceRoughness.xlsx - The surface roughness data for the three different surface types pre-fouling collected using blue-light interferometry. 

Rheometer experiments, gap height controlled by normal force (0.1 N).
2) MarineBiofilm_AmplitudeSweeps.xlsx -  Amplitude sweeps for three different surface types were ran at 1 Hz and oscillatory strain was incrementally 
	increased from 10-5 to 10-1. Raw data is presented as well as the mean +/- SD. The linear viscoelastic region (LVR) was determined for each surface.

3) MarineBiofilm_CreepData.xlsx - Creep-recovery experiments for three different surfaces were ran at different stress values, ranging from 0.5 Pa - 20 Pa. 
	Stress was applied for 60 seconds and then removed so that recovery could be measured. Shear modulus (G), viscosity (n) and elastic relaxation time 
	were calculated  from the creep-recovery curves using equations proposed in Gloag et al., 2018. The equations are presented within the workbook and
	are presented as mean +/- SD. 

4) MarineBiofilm_FrequencySweeps.xlsx - Frequency sweeps were performed for three different surfaces. Experiments were ran at an oscillation frequency of 0.1 Hz to 10Hz, 
	which equated to an angular frequency of 0.63 to 63 rad s-1. The tests were held at a constant strain of 2.5 x 10-4, which was within the LVR identified in 
	the amplitude sweep data. Raw data is presented as well as the mean +/- SD. 

5) MarineBiofilm_StructuralData.xlsx - Structural data for marine biofilms collected using Optical Coherency Tompgraphy (OCT) before and after testing on the
	rheometer. Mean thickness (mm), maximum thickness (mm), roughness coefficient (Ra*) and percent coverage (%) were calculated using custom MATLAB scripts. 
	In the workbook raw data is presented as well as the mean +/- SD. The percent differences between the before and after data are also reported. 

CRediT authorship contribution statement:
Alexandra Snowdon: Conceptualization, Methodology, Validation, Formal analysis, Investigation, Resources, Writing – original draft, Writing – review & editing, Visualization. 
Simon Dennington: Methodology, Writing – original draft, Writing – review & editing, Supervision.
Jennifer Longyear: Conceptualization, Methodology, Resources, Writing – original draft, Writing – review & editing, Supervision, Funding acquisition. 
Julian Wharton: Conceptualization, Methodology, Writing – original draft, Writing – review & editing, Supervision, Funding acquisition. 
Paul Stoodley: Conceptualization, Methodology, Writing – original draft, Writing – review & editing, Supervision, Funding acquisition.
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