Deep tissue imaging with multiphoton microscopy in the short-wavelength infrared windows
Deep tissue imaging with multiphoton microscopy in the short-wavelength infrared windows
Multiphoton microscopies are an invaluable tool in biomedical imaging given their inherent capabilities for label free imaging, optical sectioning, chemical and structural specificity. They comprise various types of Coherent Raman microscopies (CR), such as Coherent Anti-Stokes Raman Scattering (CARS), Stimulated Raman Loss (SRL) or Stimulated Raman Gain, different kinds of Harmonic Generation imaging (HG) such as Second and Third Harmonic Generation (SHG and THG respectively), and Multiphoton Autofluorescence imaging (MA) such as Two and Three Photon Excited Autofluorescence (TPEAF and ThPEAF respectively). Despite their significant advantages, multiphoton microscopies, comparably to all other types of optical microscopies, exhibit limited penetration depth in tissue due to absorption and scattering. In this work we explore the advantages of multiphoton microscopies in hard and soft deep tissue imaging when using excitation wavelengths in the range of Short-Wavelength Infrared (SWIR) windows which occur between 1000 nm and 2500 nm. These spectral windows have notable merits including longer attenuation lengths and none or very low signal absorption observed for almost all kinds of multiphoton microscopy. We show results of using excitations in the SWIR windows, generated by standard as well as novel sources, such as a thulium fibre laser, in different types of multiphoton microscopy on a variety of hard and soft tissue samples (bone, cartilage and other tissue types) and demonstrate the advantages of using excitations in this wavelength range, including longer penetration depth and high resolution for deep tissue imaging.
Society of Photo-Optical Instrumentation Engineers
Bourdakos, Konstantinos
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Xu, Lin
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Crisford, Anna
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Xu, Duanyang
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Abughazaleh, Ibrahim
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Johnson, Peter B.
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Cook, Hiroki Marius Oswald
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Srisamran, Panuwat
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Oreffo, Richard
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Richardson, David J.
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Mahajan, Sumeet
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2023
Bourdakos, Konstantinos
83f6fc3a-db12-476b-9a78-4aad8756f82f
Xu, Lin
b887cecd-d21e-49f4-9b45-6909a7369e84
Crisford, Anna
135675e1-a172-4d93-989b-93d1efb022c3
Xu, Duanyang
fa113964-baa4-41c9-b11f-b4879d001cf9
Abughazaleh, Ibrahim
73ee2e1a-fdf0-473c-8657-675ae7d73885
Johnson, Peter B.
01e8bf5b-9ab6-4403-8281-93879dfdf084
Cook, Hiroki Marius Oswald
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Srisamran, Panuwat
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Oreffo, Richard
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Richardson, David J.
ebfe1ff9-d0c2-4e52-b7ae-c1b13bccdef3
Mahajan, Sumeet
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Bourdakos, Konstantinos, Xu, Lin, Crisford, Anna, Xu, Duanyang, Abughazaleh, Ibrahim, Johnson, Peter B., Cook, Hiroki Marius Oswald, Srisamran, Panuwat, Oreffo, Richard, Richardson, David J. and Mahajan, Sumeet
(2023)
Deep tissue imaging with multiphoton microscopy in the short-wavelength infrared windows.
Mahajan, Sumeet
(ed.)
In Frontiers in Biophotonics and Imaging II.
vol. 12333,
Society of Photo-Optical Instrumentation Engineers.
9 pp
.
(doi:10.1117/12.2647553).
Record type:
Conference or Workshop Item
(Paper)
Abstract
Multiphoton microscopies are an invaluable tool in biomedical imaging given their inherent capabilities for label free imaging, optical sectioning, chemical and structural specificity. They comprise various types of Coherent Raman microscopies (CR), such as Coherent Anti-Stokes Raman Scattering (CARS), Stimulated Raman Loss (SRL) or Stimulated Raman Gain, different kinds of Harmonic Generation imaging (HG) such as Second and Third Harmonic Generation (SHG and THG respectively), and Multiphoton Autofluorescence imaging (MA) such as Two and Three Photon Excited Autofluorescence (TPEAF and ThPEAF respectively). Despite their significant advantages, multiphoton microscopies, comparably to all other types of optical microscopies, exhibit limited penetration depth in tissue due to absorption and scattering. In this work we explore the advantages of multiphoton microscopies in hard and soft deep tissue imaging when using excitation wavelengths in the range of Short-Wavelength Infrared (SWIR) windows which occur between 1000 nm and 2500 nm. These spectral windows have notable merits including longer attenuation lengths and none or very low signal absorption observed for almost all kinds of multiphoton microscopy. We show results of using excitations in the SWIR windows, generated by standard as well as novel sources, such as a thulium fibre laser, in different types of multiphoton microscopy on a variety of hard and soft tissue samples (bone, cartilage and other tissue types) and demonstrate the advantages of using excitations in this wavelength range, including longer penetration depth and high resolution for deep tissue imaging.
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Photonex2022_manuscript_KNB
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123330E
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e-pub ahead of print date: 11 January 2023
Published date: 2023
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Venue - Dates:
SPIE PHOTONEX 2022: Frontiers in Biophotonics and Imaging II, , Birmingham, United Kingdom, 2022-12-06 - 2022-12-09
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Local EPrints ID: 478020
URI: http://eprints.soton.ac.uk/id/eprint/478020
ISSN: 0277-786X
PURE UUID: 45a5a23f-8ffd-4147-8094-2e90622d14a3
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Date deposited: 19 Jun 2023 17:02
Last modified: 17 Mar 2024 03:39
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Contributors
Author:
Konstantinos Bourdakos
Author:
Ibrahim Abughazaleh
Author:
Peter B. Johnson
Author:
Hiroki Marius Oswald Cook
Author:
Panuwat Srisamran
Editor:
Sumeet Mahajan
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