READ ME File For 'Remote Ischaemic Conditioning as a Novel Therapy for Necrotising Enterocolitis'

Dataset DOI: 10.5258/SOTON/D2426

ReadMe Author: Ian Jones, University of Southampton ORCID ID : 0000-0002-5161-2096

This dataset supports the thesis entitled REMOTE ISCHAEMIC CONDITIONING AS A NOVEL THERAPY FOR NECROTISING ENTEROCOLITIS
AWARDED BY: Univeristy of Southampton
DATE OF AWARD: 2022

DESCRIPTION OF THE DATA
There are essentially three datasets for this thesis. The first relates to a systematic review of NEC outcomes (Chapter 2) as consists of a single Microsoft Excel 
file with the details of the included papers and the data extracted from them.

The second relates to the primary research using an animal model of NEC. Each datafile is a Microsoft Excel file and contains multiple datapoints from each animal;
including the macroscopic assessment of injury, microscopic assessment, using the Chiu-Park scoring system, serum cytokine analysis, and myeloperoxidase activity. In
Each case, the individual animal is identified by a unique, randomly generated 3-digit code. (Chapters 5-8 and 10).

The third set is the raw count from RNAseq transciptomics analysis of the intestine of animals in protocol 1a (plus controls). The datafile is a .csv file.



This dataset contains:
1.	Systematic Review of Outcomes for Necrotising Enterocolitis
 - NEC_Outcomes_Systematic_Review_Dataset.xlxs

2.	Animal Experiments
 - Zip File: Animal_Work_Dataset.zip

PROTOCOL	DESCRIPTION										FILENAME
Controls	IRI  and SHAM controls									Protocol_controls_-_IRI_&_SHAM.xlsx
1a		Remote Ischaemic pre-Conditioning delivered with a ligature				Protocol_1.xlsx
1b		Remote Ischaemic pre-Conditioning delivered with a rodent blood pressure cuff		Protocol_1.xlsx
2		Remote Ischaemic post-Conditioning							Protocol_2.xlsx
3a		Remote Ischaemic pre-Conditioning (Early pre-conditioning)				Protocol_3.xlsx
3b		Remote Ischaemic pre-Conditioning (Early and immediate pre-conditioning)		Protocol_3.xlsx
4		Maternal Remote Ischaemic Conditioning							Protocol_4.xlsx

3.	RNAseq Transcriptomics Data
Raw_Counts_All_Samples.csv





Date of data collection: 2017-2020


Related projects/Funders:
This work was part-funded by a Research Fellowship from the Royal College of Surgeons of England

Related publication:
Jones, I.H. and Hall N.J., Contemporary Outcomes for Infants with Necrotizing Enterocolitis-A Systematic Review.  J Pediatr 2020;220:86-92

Date that the file was created: November 2022

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