Dissecting the relative contribution of ECA3 and group 8/9 cation diffusion facilitators to manganese homeostasis in Arabidopsis thaliana
Dissecting the relative contribution of ECA3 and group 8/9 cation diffusion facilitators to manganese homeostasis in Arabidopsis thaliana
Manganese (Mn) is an essential micronutrient for plant growth but becomes toxic when present in excess. A number of Arabidopsis proteins are involved in Mn transport including ECA3, MTPs, and NRAMPs; however, their relative contributions to Mn homeostasis remain to be demonstrated. A major focus here was to clarify the importance of ECA3 in responding to Mn deficiency and toxicity using a range of mutants. We show that ECA3 localizes to the trans-Golgi and plays a major role in response to Mn deficiency with severe effects seen in eca3 nramp1 nramp2 under low Mn supply. ECA3 plays a minor role in Mn-toxicity tolerance, but only when the cis-Golgi-localized MTP11 is non-functional. We also use mutants and overexpressors to determine the relative contributions of MTP members to Mn homeostasis. The trans-Golgi-localized MTP10 plays a role in Mn-toxicity tolerance, but this is only revealed in mutants when MTP8 and MTP11 are non-functional and when overexpressed in mtp11 mutants. MTP8 and MTP10 confer greater Mn-toxicity resistance to the pmr1 yeast mutant than MTP11, and an important role for the first aspartate in the fifth transmembrane domain DxxxD motif is demonstrated. Overall, new insight into the relative influence of key transporters in Mn homeostasis is provided.
P-type ATPase, cation diffusion facilitator (CDF), heavy metal, manganese, metal tolerance protein (MTP), natural resistance-associated macrophage protein (NRAMP), transport
Farthing, Emily C.
121f4586-6ec7-4ee4-af80-97f45ebba4dd
Henbest, Kate C.
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Garcia-Becerra, Tania
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Peaston, Kerry A.
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Williams, Lorraine E.
79ee1856-3732-492b-8ac5-239749c85d9e
22 May 2023
Farthing, Emily C.
121f4586-6ec7-4ee4-af80-97f45ebba4dd
Henbest, Kate C.
be402ef6-ddf6-4180-9a66-39a824016422
Garcia-Becerra, Tania
3afa9c47-be57-4dc5-99ea-6b1e05d99d1f
Peaston, Kerry A.
3b85d38d-b038-4456-861f-d43f2c7fc6ee
Williams, Lorraine E.
79ee1856-3732-492b-8ac5-239749c85d9e
Farthing, Emily C., Henbest, Kate C., Garcia-Becerra, Tania, Peaston, Kerry A. and Williams, Lorraine E.
(2023)
Dissecting the relative contribution of ECA3 and group 8/9 cation diffusion facilitators to manganese homeostasis in Arabidopsis thaliana.
Plant Direct, 7 (5), [e495].
(doi:10.1002/pld3.495).
Abstract
Manganese (Mn) is an essential micronutrient for plant growth but becomes toxic when present in excess. A number of Arabidopsis proteins are involved in Mn transport including ECA3, MTPs, and NRAMPs; however, their relative contributions to Mn homeostasis remain to be demonstrated. A major focus here was to clarify the importance of ECA3 in responding to Mn deficiency and toxicity using a range of mutants. We show that ECA3 localizes to the trans-Golgi and plays a major role in response to Mn deficiency with severe effects seen in eca3 nramp1 nramp2 under low Mn supply. ECA3 plays a minor role in Mn-toxicity tolerance, but only when the cis-Golgi-localized MTP11 is non-functional. We also use mutants and overexpressors to determine the relative contributions of MTP members to Mn homeostasis. The trans-Golgi-localized MTP10 plays a role in Mn-toxicity tolerance, but this is only revealed in mutants when MTP8 and MTP11 are non-functional and when overexpressed in mtp11 mutants. MTP8 and MTP10 confer greater Mn-toxicity resistance to the pmr1 yeast mutant than MTP11, and an important role for the first aspartate in the fifth transmembrane domain DxxxD motif is demonstrated. Overall, new insight into the relative influence of key transporters in Mn homeostasis is provided.
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Dissecting the relative contribution of ECA3
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Accepted/In Press date: 27 March 2023
Published date: 22 May 2023
Additional Information:
Funding Information:
We gratefully acknowledge Dr. Jinbo Shen (The Chinese University of Hong Kong) for the ManI cis-Golgi marker, Dr. John Runions (Oxford Brookes University, UK) for N. tabacum Petit Havana plants, and Professor Ute Kramer (Ruhr University, Bochum) for the zrc1 cot1 strain. We thank L.F.G Mendoza for technical help. Research into endomembrane transport in the LEW lab is supported by Biotechnology and Biological Sciences Research Council (BB/L010313/1) and the Gerald Kerkut Trust.
Funding Information:
We gratefully acknowledge Dr. Jinbo Shen (The Chinese University of Hong Kong) for the ManI Golgi marker, Dr. John Runions (Oxford Brookes University, UK) for Petit Havana plants, and Professor Ute Kramer (Ruhr University, Bochum) for the strain. We thank L.F.G Mendoza for technical help. Research into endomembrane transport in the LEW lab is supported by Biotechnology and Biological Sciences Research Council (BB/L010313/1) and the Gerald Kerkut Trust. cis‐ N. tabacum zrc1 cot1
Publisher Copyright:
© 2023 The Authors. Plant Direct published by American Society of Plant Biologists and the Society for Experimental Biology and John Wiley & Sons Ltd.
Keywords:
P-type ATPase, cation diffusion facilitator (CDF), heavy metal, manganese, metal tolerance protein (MTP), natural resistance-associated macrophage protein (NRAMP), transport
Identifiers
Local EPrints ID: 478847
URI: http://eprints.soton.ac.uk/id/eprint/478847
PURE UUID: 9d746132-36c1-4d09-a9a2-58f7825d6670
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Date deposited: 11 Jul 2023 17:04
Last modified: 17 Mar 2024 03:23
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Contributors
Author:
Emily C. Farthing
Author:
Kate C. Henbest
Author:
Tania Garcia-Becerra
Author:
Kerry A. Peaston
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