Native mass spectrometry goes more native: investigation of membrane protein complexes directly from SMALPs
Native mass spectrometry goes more native: investigation of membrane protein complexes directly from SMALPs
Other than more widely used methods, the use of styrene maleic acid allows the direct extraction of membrane proteins from the lipid bilayer into SMALPs keeping it in its native lipid surrounding. Here we present the combined use of SMALPs and LILBID-MS, allowing determination of oligomeric states of membrane proteins of different functionality directly from the native nanodiscs.
13702-13705
Hellwig, Nils
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Peetz, Oliver
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Ahdash, Zainab
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Tascon, Igor
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Booth, Paula J.
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Mikusevic, Vedrana
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Diskowski, Marina
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Politis, Argyris
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Hellmich, Yvonne
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Hanelt, Inga
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Reading, Eamonn
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Morgner, Nina
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19 November 2018
Hellwig, Nils
48c17a38-4bb4-439a-9ca9-af27e3aad837
Peetz, Oliver
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Ahdash, Zainab
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Tascon, Igor
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Booth, Paula J.
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Mikusevic, Vedrana
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Diskowski, Marina
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Politis, Argyris
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Hellmich, Yvonne
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Hanelt, Inga
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Reading, Eamonn
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Morgner, Nina
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Hellwig, Nils, Peetz, Oliver, Ahdash, Zainab, Tascon, Igor, Booth, Paula J., Mikusevic, Vedrana, Diskowski, Marina, Politis, Argyris, Hellmich, Yvonne, Hanelt, Inga, Reading, Eamonn and Morgner, Nina
(2018)
Native mass spectrometry goes more native: investigation of membrane protein complexes directly from SMALPs.
Chemical communications (Cambridge, England), 54, .
(doi:10.1039/c8cc06284f).
Abstract
Other than more widely used methods, the use of styrene maleic acid allows the direct extraction of membrane proteins from the lipid bilayer into SMALPs keeping it in its native lipid surrounding. Here we present the combined use of SMALPs and LILBID-MS, allowing determination of oligomeric states of membrane proteins of different functionality directly from the native nanodiscs.
Text
c8cc06284f
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More information
Accepted/In Press date: 6 September 2018
Published date: 19 November 2018
Identifiers
Local EPrints ID: 478888
URI: http://eprints.soton.ac.uk/id/eprint/478888
ISSN: 1359-7345
PURE UUID: eb9bd69c-db4c-4588-8c34-5fab69838968
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Date deposited: 12 Jul 2023 16:39
Last modified: 17 Mar 2024 04:19
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Contributors
Author:
Nils Hellwig
Author:
Oliver Peetz
Author:
Zainab Ahdash
Author:
Igor Tascon
Author:
Paula J. Booth
Author:
Vedrana Mikusevic
Author:
Marina Diskowski
Author:
Argyris Politis
Author:
Yvonne Hellmich
Author:
Inga Hanelt
Author:
Eamonn Reading
Author:
Nina Morgner
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