Detection of membrane proteins
Detection of membrane proteins
According to the present invention, there is provided a method of detecting a membrane protein by mass spectrometry, the method comprising the steps of: (a) providing a solution comprising a detergent micelle in which said membrane protein is contained, wherein said solution contains a polyoxyalkylene glycol detergent; (b) providing a mass spectrometer comprising a nanoelectrospray ionisation source, a mass analyser and a detector; (c) vaporising the solution using the nanoelectrospray ionisation source under conditions such that the membrane protein is released from the micelle; (d) ionising the membrane protein; (e) resolving the ionised membrane protein using the mass analyser; and (f) detecting the resolved membrane protein using the detector. Also provided are reagents for use in said method.
US20150346214A1
United States Patent and Trademark Office
3 December 2015
Laganowsky, Arthur
bb7b8bdf-2337-4aed-a806-66fc26697439
Reading, Eamonn
62fed933-f867-4c72-89e7-83aea573a836
Laganowsky, Arthur and Reading, Eamonn
(Inventors)
(2015)
Detection of membrane proteins.
US20150346214A1.
Abstract
According to the present invention, there is provided a method of detecting a membrane protein by mass spectrometry, the method comprising the steps of: (a) providing a solution comprising a detergent micelle in which said membrane protein is contained, wherein said solution contains a polyoxyalkylene glycol detergent; (b) providing a mass spectrometer comprising a nanoelectrospray ionisation source, a mass analyser and a detector; (c) vaporising the solution using the nanoelectrospray ionisation source under conditions such that the membrane protein is released from the micelle; (d) ionising the membrane protein; (e) resolving the ionised membrane protein using the mass analyser; and (f) detecting the resolved membrane protein using the detector. Also provided are reagents for use in said method.
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Published date: 3 December 2015
Identifiers
Local EPrints ID: 478975
URI: http://eprints.soton.ac.uk/id/eprint/478975
PURE UUID: 316986db-4872-4459-bcd9-851ef805f2a1
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Date deposited: 17 Jul 2023 16:32
Last modified: 17 Mar 2024 04:19
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Contributors
Inventor:
Arthur Laganowsky
Inventor:
Eamonn Reading
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