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Dye displacement assay for saccharides using benzoxaborole hydrogels

Dye displacement assay for saccharides using benzoxaborole hydrogels
Dye displacement assay for saccharides using benzoxaborole hydrogels
Dye displacement assays are a simple but effective method to determine the concentration of target analytes. Previously, we have shown that phenylboronic acid pinacol ester hydrogels (borogels) can be used to develop a boronic acid–Alizarin red S dye displacement assay for the determination of fructose (orange to red). In this work, benzoxaborole hydrogels (BOBgels) were developed, and these BOBgels demonstrated an enhanced apparent binding affinity towards monosaccharides, in particular towards glucose.
2191-1363
266-268
Lampard, Emma V.
c3f39fc0-d5b4-47be-b8d2-0787c7e3a5b5
Sedgwick, Adam C.
7329dac0-8976-4885-bbe9-6edb9d1ab017
Sombuttan, Thitima
48afdf07-27de-478c-b7ba-60f6e09d7da2
Williams, George
26810522-92ef-4b61-a766-582bf15be280
Wannalerse, Boontana
974876cc-a40e-44d9-bbd8-48eaa4a316e3
Jenkins, A. Toby A.
e0e4ebe3-c2d6-4aae-81eb-66923f6f98dd
Bull, Steven D.
6f8a4a6d-a26d-4fba-bb20-08d48d8ea144
James, Tony D.
5cfd8c35-9fa1-4583-934e-5bc6961f6fa5
Lampard, Emma V.
c3f39fc0-d5b4-47be-b8d2-0787c7e3a5b5
Sedgwick, Adam C.
7329dac0-8976-4885-bbe9-6edb9d1ab017
Sombuttan, Thitima
48afdf07-27de-478c-b7ba-60f6e09d7da2
Williams, George
26810522-92ef-4b61-a766-582bf15be280
Wannalerse, Boontana
974876cc-a40e-44d9-bbd8-48eaa4a316e3
Jenkins, A. Toby A.
e0e4ebe3-c2d6-4aae-81eb-66923f6f98dd
Bull, Steven D.
6f8a4a6d-a26d-4fba-bb20-08d48d8ea144
James, Tony D.
5cfd8c35-9fa1-4583-934e-5bc6961f6fa5

Lampard, Emma V., Sedgwick, Adam C., Sombuttan, Thitima, Williams, George, Wannalerse, Boontana, Jenkins, A. Toby A., Bull, Steven D. and James, Tony D. (2018) Dye displacement assay for saccharides using benzoxaborole hydrogels. ChemistryOpen, 7 (3), 266-268. (doi:10.1002/open.201700193).

Record type: Article

Abstract

Dye displacement assays are a simple but effective method to determine the concentration of target analytes. Previously, we have shown that phenylboronic acid pinacol ester hydrogels (borogels) can be used to develop a boronic acid–Alizarin red S dye displacement assay for the determination of fructose (orange to red). In this work, benzoxaborole hydrogels (BOBgels) were developed, and these BOBgels demonstrated an enhanced apparent binding affinity towards monosaccharides, in particular towards glucose.

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Published date: 30 January 2018

Identifiers

Local EPrints ID: 479305
URI: http://eprints.soton.ac.uk/id/eprint/479305
ISSN: 2191-1363
PURE UUID: 0875d2ce-3f00-45dd-a044-a7ccbccc6bce
ORCID for George Williams: ORCID iD orcid.org/0000-0001-6162-8895

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Date deposited: 20 Jul 2023 16:54
Last modified: 17 Mar 2024 04:17

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Contributors

Author: Emma V. Lampard
Author: Adam C. Sedgwick
Author: Thitima Sombuttan
Author: George Williams ORCID iD
Author: Boontana Wannalerse
Author: A. Toby A. Jenkins
Author: Steven D. Bull
Author: Tony D. James

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