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Expression and localization of lung surfactant protein A in human tissues

Madsen, Jens, Tornøe, Ida, Nielsen, Ole, Koch, Claus, Steinhilber, Wolfram and Holmskov, Uffe (2003) Expression and localization of lung surfactant protein A in human tissues American Journal of Respiratory Cell and Molecular Biology, 29, (5), pp. 591-597. (doi:10.1165/rcmb.2002-0274OC).

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Lung surfactant protein A (SP-A) is a collectin produced by alveolar type II cells and Clara cells. It binds to carbohydrate structures on microorganisms, initiating effector mechanisms of innate immunity and modulating the inflammatory response in the lung. Reverse transcriptase-polymerase chain reaction was performed on a panel of RNAs from human tissues for SP-A mRNA expression. The lung was the main site of synthesis, but transcripts were readily amplified from the trachea, prostate, pancreas, and thymus. Weak expression was observed in the colon and salivary gland. SP-A sequences derived from lung and thymus mRNA revealed the presence of both SP-A1 and SP-A2, whereas only SP-A2 expression was found in the trachea and prostate. Monoclonal antibodies were raised against SP-A and characterized. One of these (HYB 238-4) reacted in Western blotting with both reduced and unreduced SP-A, with N-deglycosylated and collagenase-treated SP-A, and with both recombinant SP-A1 and SP-A2. This antibody was used to demonstrate SP-A in immunohistochemistry of human tissues. Strong SP-A immunoreactivity was seen in alveolar type-II cells, Clara cells, and on and within alveolar macrophages, but no extrapulmonary SP-A immunoreactivity was observed. In contrast to lung surfactant protein D (SP-D), which is generally expressed on mucosal surfaces, SP-A seems to be restricted to the respiratory system.

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Published date: November 2003


Local EPrints ID: 47971
ISSN: 1044-1549
PURE UUID: 312bb65a-f5dc-48d6-b513-124a46a677da
ORCID for Jens Madsen: ORCID iD

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Date deposited: 16 Aug 2007
Last modified: 17 Jul 2017 15:01

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Author: Jens Madsen ORCID iD
Author: Ida Tornøe
Author: Ole Nielsen
Author: Claus Koch
Author: Wolfram Steinhilber
Author: Uffe Holmskov

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