Genomic and molecular characterization of bovine surfactant protein D (SP-D)
Genomic and molecular characterization of bovine surfactant protein D (SP-D)
Collectins are a group of C-type lectins involved in the innate host defense against pathogens. They selectively recognize non-self glyco-conjugates on the surface of microorganisms and induce lysis, agglutination, and phagocytosis to eliminate invading microorganisms. With the perspective of being able to identify surfactant protein D (SP-D) polymorphisms associated with immune-compromised phenotypes in cattle, we have characterized the gene encoding bovine SP-D and its proximal promoter. Cloning and sequencing of the bSP-D gene, including the complete 5'-untranslated sequence, reveal that the gene comprises nine exons spanning approximately 10.5 kb with an organization resembling the bovine conglutinin gene. The gene localizes to the same locus as the conglutinin gene on Bos taurus chromosome 28 at position q1.8, which also includes the genes encoding CL-43 and CL-46. Several potential cis-regulatory elements, similar to elements known to regulate the transcription of human SP-D, were identified in the 5'-upstream sequence. RT-PCR analysis revealed that bovine SP-D is heavily expressed in the lung and the trachea, but also in segments of the gastrointestinal tract, the mammary glands and the salivary glands. By genotyping we assigned two potential polymorphisms leading to variations in the amino acid composition of the carbohydrate recognition domain (242 Glu/Val and 268 Ala/Gly).
collectin, SP-D, innate immunity, comparative immunology
369-376
Gjerstorff, Morten
ddb61403-ec79-4e62-8fde-e6ec691603fd
Madsen, Jens
b5d8ae35-00ac-4d19-930e-d8ddec497359
Bendixen, Christian
73dc6de4-4523-4c85-9705-a67386f1dd47
Holmskov, Uffe
8a55aecd-694c-4d61-849b-9cf32da8ba39
Hansen, Soren
a97e00fd-2d4a-4959-ad73-43a7f8744303
June 2004
Gjerstorff, Morten
ddb61403-ec79-4e62-8fde-e6ec691603fd
Madsen, Jens
b5d8ae35-00ac-4d19-930e-d8ddec497359
Bendixen, Christian
73dc6de4-4523-4c85-9705-a67386f1dd47
Holmskov, Uffe
8a55aecd-694c-4d61-849b-9cf32da8ba39
Hansen, Soren
a97e00fd-2d4a-4959-ad73-43a7f8744303
Gjerstorff, Morten, Madsen, Jens, Bendixen, Christian, Holmskov, Uffe and Hansen, Soren
(2004)
Genomic and molecular characterization of bovine surfactant protein D (SP-D).
Molecular Immunology, 41 (4), .
(doi:10.1016/j.molimm.2004.03.005).
Abstract
Collectins are a group of C-type lectins involved in the innate host defense against pathogens. They selectively recognize non-self glyco-conjugates on the surface of microorganisms and induce lysis, agglutination, and phagocytosis to eliminate invading microorganisms. With the perspective of being able to identify surfactant protein D (SP-D) polymorphisms associated with immune-compromised phenotypes in cattle, we have characterized the gene encoding bovine SP-D and its proximal promoter. Cloning and sequencing of the bSP-D gene, including the complete 5'-untranslated sequence, reveal that the gene comprises nine exons spanning approximately 10.5 kb with an organization resembling the bovine conglutinin gene. The gene localizes to the same locus as the conglutinin gene on Bos taurus chromosome 28 at position q1.8, which also includes the genes encoding CL-43 and CL-46. Several potential cis-regulatory elements, similar to elements known to regulate the transcription of human SP-D, were identified in the 5'-upstream sequence. RT-PCR analysis revealed that bovine SP-D is heavily expressed in the lung and the trachea, but also in segments of the gastrointestinal tract, the mammary glands and the salivary glands. By genotyping we assigned two potential polymorphisms leading to variations in the amino acid composition of the carbohydrate recognition domain (242 Glu/Val and 268 Ala/Gly).
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Published date: June 2004
Keywords:
collectin, SP-D, innate immunity, comparative immunology
Identifiers
Local EPrints ID: 47973
URI: http://eprints.soton.ac.uk/id/eprint/47973
ISSN: 0161-5890
PURE UUID: f748395f-dd95-4c71-b6ba-e36f825d4499
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Date deposited: 15 Aug 2007
Last modified: 16 Mar 2024 03:56
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Contributors
Author:
Morten Gjerstorff
Author:
Jens Madsen
Author:
Christian Bendixen
Author:
Uffe Holmskov
Author:
Soren Hansen
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