Cloning and characterisation of two new cDNAs encoding murine triple LIM domains
Cloning and characterisation of two new cDNAs encoding murine triple LIM domains
We have cloned and sequenced two new cDNAs that code for proteins carrying the related triple LIM domains (acronym of Lin-11, Isl-1, Mec-3) proteins. These LIM domains show good agreement to the LIM domain consensus sequence, but also exhibit some novel variations. The 1.36-and 2.8-kb cDNAs are probably splice variants of one gene and code for 42- and 50-kDa proteins, respectively. The larger transcript has a 900-nucleotide (nt) 3' untranslated region (UTR). High levels of the 2.8-kb transcript can be detected in many tissues, and all tissues show some level of expression of both transcripts, the larger transcript being more abundant. In adult testis there are very high levels of the 1.36-kb transcript and moderate levels of the 2.8-kb transcript. The wide tissue distribution and high levels of expression suggest an important role for these proteins in cellular function.
Amino Acid Sequence Animals Base Sequence *Caenorhabditis elegans Proteins Consensus Sequence Cytoskeletal Proteins DNA, Complementary DNA-Binding Proteins/*genetics Gene Library Helminth Proteins *Homeodomain Proteins LIM-Homeodomain Proteins Male Mice Molecular Sequence Data *Nerve Tissue Proteins RNA, Messenger/genetics RNA-Binding Proteins Repetitive Sequences, Nucleic Acid/*genetics Sequence Analysis, DNA Sequence Homology, Amino Acid Tissue Distribution Transcription Factors
283-286
Divecha, N.
5c2ad0f8-4ce7-405f-8a15-2fc4ab96d787
Charleston, B.
896c65c1-1ad3-43ec-ab5a-cca7310b642b
1995
Divecha, N.
5c2ad0f8-4ce7-405f-8a15-2fc4ab96d787
Charleston, B.
896c65c1-1ad3-43ec-ab5a-cca7310b642b
Divecha, N. and Charleston, B.
(1995)
Cloning and characterisation of two new cDNAs encoding murine triple LIM domains.
Gene, 156 (2), .
(doi:10.1016/0378-1119(95)00088-n).
Abstract
We have cloned and sequenced two new cDNAs that code for proteins carrying the related triple LIM domains (acronym of Lin-11, Isl-1, Mec-3) proteins. These LIM domains show good agreement to the LIM domain consensus sequence, but also exhibit some novel variations. The 1.36-and 2.8-kb cDNAs are probably splice variants of one gene and code for 42- and 50-kDa proteins, respectively. The larger transcript has a 900-nucleotide (nt) 3' untranslated region (UTR). High levels of the 2.8-kb transcript can be detected in many tissues, and all tissues show some level of expression of both transcripts, the larger transcript being more abundant. In adult testis there are very high levels of the 1.36-kb transcript and moderate levels of the 2.8-kb transcript. The wide tissue distribution and high levels of expression suggest an important role for these proteins in cellular function.
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Published date: 1995
Keywords:
Amino Acid Sequence Animals Base Sequence *Caenorhabditis elegans Proteins Consensus Sequence Cytoskeletal Proteins DNA, Complementary DNA-Binding Proteins/*genetics Gene Library Helminth Proteins *Homeodomain Proteins LIM-Homeodomain Proteins Male Mice Molecular Sequence Data *Nerve Tissue Proteins RNA, Messenger/genetics RNA-Binding Proteins Repetitive Sequences, Nucleic Acid/*genetics Sequence Analysis, DNA Sequence Homology, Amino Acid Tissue Distribution Transcription Factors
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Local EPrints ID: 480033
URI: http://eprints.soton.ac.uk/id/eprint/480033
ISSN: 0378-1119
PURE UUID: 92737a03-f36a-4650-b5c4-7e000d0410d5
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Date deposited: 01 Aug 2023 16:37
Last modified: 17 Mar 2024 02:59
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Author:
B. Charleston
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