Phosphoinositide signalling enzymes in rat liver nuclei: phosphoinositidase C isoform beta 1 is specifically, but not predominantly, located in the nucleus
Phosphoinositide signalling enzymes in rat liver nuclei: phosphoinositidase C isoform beta 1 is specifically, but not predominantly, located in the nucleus
The presence of phosphoinositide-mobilizing enzymes has been investigated in purified rat liver nuclei by radiolabelling and by probing with antibodies. A Ca(2+)-activated phosphoinositidase C (PIC) is present and was shown immunologically to be the beta 1 isoform. No gamma- or delta-PIC was found. However, only 5% of the total beta 1-PIC isoform is nuclear, with the majority being cytosolic. G alpha q and G alpha 11, the suggested physiological activators of beta 1-PIC, were not present. A PtdIns4P 5-kinase is also present, which immunologically is shown to be the C isoform. All of these nuclear inositide enzymes still remained after the removal of the nuclear envelope with Triton X-100, consistent with the concept of an intranuclear inositide cycle [Divecha, Banfic and Irvine (1991) EMBO. J. 10, 3207-3214].
Animals Calcium/pharmacology *Cell Compartmentation Cell Nucleus/enzymology Cytosol/enzymology Isoenzymes/*analysis/drug effects/immunology/metabolism Liver/*enzymology Male Nuclear Envelope Phosphatidylinositols/metabolism Phosphoric Diester Hydrolases/*analysis/drug effects/immunology/metabolism Rats Rats, Inbred Strains Rats, Wistar Subcellular Fractions/enzymology
617-620
Divecha, N.
5c2ad0f8-4ce7-405f-8a15-2fc4ab96d787
Rhee, S. G.
0f2b63da-34a7-4910-b00e-ec4b67c596dc
Letcher, A. J.
08f1eb2a-f4b2-4b38-aa51-3ac65762e3d3
Irvine, R. F.
a8a94b1b-419c-4262-b745-eae1941ce145
1993
Divecha, N.
5c2ad0f8-4ce7-405f-8a15-2fc4ab96d787
Rhee, S. G.
0f2b63da-34a7-4910-b00e-ec4b67c596dc
Letcher, A. J.
08f1eb2a-f4b2-4b38-aa51-3ac65762e3d3
Irvine, R. F.
a8a94b1b-419c-4262-b745-eae1941ce145
Divecha, N., Rhee, S. G., Letcher, A. J. and Irvine, R. F.
(1993)
Phosphoinositide signalling enzymes in rat liver nuclei: phosphoinositidase C isoform beta 1 is specifically, but not predominantly, located in the nucleus.
Biochemical Journal, 289 (3), .
(doi:10.1042/bj2890617).
Abstract
The presence of phosphoinositide-mobilizing enzymes has been investigated in purified rat liver nuclei by radiolabelling and by probing with antibodies. A Ca(2+)-activated phosphoinositidase C (PIC) is present and was shown immunologically to be the beta 1 isoform. No gamma- or delta-PIC was found. However, only 5% of the total beta 1-PIC isoform is nuclear, with the majority being cytosolic. G alpha q and G alpha 11, the suggested physiological activators of beta 1-PIC, were not present. A PtdIns4P 5-kinase is also present, which immunologically is shown to be the C isoform. All of these nuclear inositide enzymes still remained after the removal of the nuclear envelope with Triton X-100, consistent with the concept of an intranuclear inositide cycle [Divecha, Banfic and Irvine (1991) EMBO. J. 10, 3207-3214].
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Published date: 1993
Keywords:
Animals Calcium/pharmacology *Cell Compartmentation Cell Nucleus/enzymology Cytosol/enzymology Isoenzymes/*analysis/drug effects/immunology/metabolism Liver/*enzymology Male Nuclear Envelope Phosphatidylinositols/metabolism Phosphoric Diester Hydrolases/*analysis/drug effects/immunology/metabolism Rats Rats, Inbred Strains Rats, Wistar Subcellular Fractions/enzymology
Identifiers
Local EPrints ID: 480106
URI: http://eprints.soton.ac.uk/id/eprint/480106
ISSN: 0264-6021
PURE UUID: 64368c37-5de7-4375-bed5-f1a3f92c8431
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Date deposited: 01 Aug 2023 16:49
Last modified: 17 Mar 2024 02:59
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Author:
S. G. Rhee
Author:
A. J. Letcher
Author:
R. F. Irvine
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