Molecular species analysis of 1,2-diacylglycerols and phosphatidic acid formed during bombesin stimulation of Swiss 3T3 cells
Molecular species analysis of 1,2-diacylglycerols and phosphatidic acid formed during bombesin stimulation of Swiss 3T3 cells
Swiss 3T3 cells were labelled with [3H]glycerol and stimulated with bombesin over a time course of 20 min. The individual 1,2-diacylglycerols produced were quantified by acetylation followed by analysis by HPLC and argentation chromatography. The major phospholipids and phosphatidic acid were acetolysed and then analysed in the same manner. The data show that even at an early time of stimulation (30 s), stimulated diacylglycerol formation comes from at least two sources--phosphoinositides and phosphatidylcholine.
184-188
Divecha, N.
5c2ad0f8-4ce7-405f-8a15-2fc4ab96d787
Lander, David J.
f47abaf9-3566-48be-98e2-824d9583a77c
Scott, Trevor W.
053ee5f0-0aff-4c36-a157-ef9a4bc48fa2
Irvine, R. F.
a8a94b1b-419c-4262-b745-eae1941ce145
1991
Divecha, N.
5c2ad0f8-4ce7-405f-8a15-2fc4ab96d787
Lander, David J.
f47abaf9-3566-48be-98e2-824d9583a77c
Scott, Trevor W.
053ee5f0-0aff-4c36-a157-ef9a4bc48fa2
Irvine, R. F.
a8a94b1b-419c-4262-b745-eae1941ce145
Divecha, N., Lander, David J., Scott, Trevor W. and Irvine, R. F.
(1991)
Molecular species analysis of 1,2-diacylglycerols and phosphatidic acid formed during bombesin stimulation of Swiss 3T3 cells.
Biochimica et Biophysica Acta (BBA) - Biomembranes, 1093 (2-3), .
(doi:10.1016/0167-4889(91)90121-d).
Abstract
Swiss 3T3 cells were labelled with [3H]glycerol and stimulated with bombesin over a time course of 20 min. The individual 1,2-diacylglycerols produced were quantified by acetylation followed by analysis by HPLC and argentation chromatography. The major phospholipids and phosphatidic acid were acetolysed and then analysed in the same manner. The data show that even at an early time of stimulation (30 s), stimulated diacylglycerol formation comes from at least two sources--phosphoinositides and phosphatidylcholine.
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Published date: 1991
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Local EPrints ID: 480110
URI: http://eprints.soton.ac.uk/id/eprint/480110
ISSN: 0006-3002
PURE UUID: ce7a3bd7-d587-49a6-a8ab-453a2c8e852a
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Date deposited: 01 Aug 2023 16:50
Last modified: 17 Mar 2024 02:59
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Author:
David J. Lander
Author:
Trevor W. Scott
Author:
R. F. Irvine
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