Methods to assess changes in the pattern of nuclear phosphoinositides
Methods to assess changes in the pattern of nuclear phosphoinositides
Phosphatidylinositol (PtdIns) and its phosphorylated derivatives represent less than 5% of total membrane phospholipids in cells. Despite their low abundance, they form a dynamic signalling system that is regulated in response to a variety of extra and intra-cellular cues (Curr Opin Genet Dev 14:196-202, 2004). Phosphoinositides and the enzymes that synthesize them are found in many different sub-cellular compartments including the nuclear matrix, heterochromatin, and sites of active RNA splicing, suggesting that phosphoinositides may regulate specific functions within the nuclear compartment (Nat Rev Mol Cell Biol 4:349-360, 2003; Curr Top Microbiol Immunol 282:177-206, 2004; Cell Mol Life Sci 61:1143-1156, 2004). The existence of distinct sub-cellular pools has led to the challenging task of understanding how the different pools are regulated and how changes in the mass of lipids within the nucleus can modulate nuclear specific pathways. Here we describe methods to determine how enzymatic activities that modulate nuclear phosphoinositides are changed in response to extracellular stimuli.
Animals Cell Fractionation/methods Cell Line Cell Nucleus/chemistry/enzymology/*metabolism Isomerism Phosphates/analysis Phosphatidylinositols/chemistry/isolation & purification/*metabolism
165-177
Divecha, N.
5c2ad0f8-4ce7-405f-8a15-2fc4ab96d787
1 January 2010
Divecha, N.
5c2ad0f8-4ce7-405f-8a15-2fc4ab96d787
Divecha, N.
(2010)
Methods to assess changes in the pattern of nuclear phosphoinositides.
Methods in Molecular Biology, 645, .
(doi:10.1007/978-1-60327-175-2_11).
Abstract
Phosphatidylinositol (PtdIns) and its phosphorylated derivatives represent less than 5% of total membrane phospholipids in cells. Despite their low abundance, they form a dynamic signalling system that is regulated in response to a variety of extra and intra-cellular cues (Curr Opin Genet Dev 14:196-202, 2004). Phosphoinositides and the enzymes that synthesize them are found in many different sub-cellular compartments including the nuclear matrix, heterochromatin, and sites of active RNA splicing, suggesting that phosphoinositides may regulate specific functions within the nuclear compartment (Nat Rev Mol Cell Biol 4:349-360, 2003; Curr Top Microbiol Immunol 282:177-206, 2004; Cell Mol Life Sci 61:1143-1156, 2004). The existence of distinct sub-cellular pools has led to the challenging task of understanding how the different pools are regulated and how changes in the mass of lipids within the nucleus can modulate nuclear specific pathways. Here we describe methods to determine how enzymatic activities that modulate nuclear phosphoinositides are changed in response to extracellular stimuli.
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Published date: 1 January 2010
Additional Information:
Divecha, Nullin eng 2010/07/21 Methods Mol Biol. 2010;645:165-77. doi: 10.1007/978-1-60327-175-2_11.
Keywords:
Animals Cell Fractionation/methods Cell Line Cell Nucleus/chemistry/enzymology/*metabolism Isomerism Phosphates/analysis Phosphatidylinositols/chemistry/isolation & purification/*metabolism
Identifiers
Local EPrints ID: 480117
URI: http://eprints.soton.ac.uk/id/eprint/480117
ISSN: 1940-6029
PURE UUID: ec918f20-3cbd-4f34-89a8-fc59d891f9f3
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Date deposited: 01 Aug 2023 16:51
Last modified: 17 Mar 2024 02:59
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