Purification and characterization of phosphatidylinositol 4-phosphate 5-kinases
Purification and characterization of phosphatidylinositol 4-phosphate 5-kinases
We detail the purification and characterization of three distinct isoforms of PtdIns4P 5-kinase present in bovine brain. One of these, PtdIns4P 5-kinase C, was purified to apparent homogeneity, and SDS/PAGE analysis demonstrated a single polypeptide and molecular mass 53 KDa. These three isoforms were shown to differ in their kinetic properties, and immunological characterization with an antibody raised to PtdIns4P 5-kinase C demonstrated that this isoform was unrelated to the other two. Furthermore, PtdIns4P 5-kinase C was shown to be the bovine brain homologue of the Type II PtdIns4P 5-kinase previously purified from human erythrocytes [Bazenet, Ruano, Brockman & Anderson (1990) J. Biol. Chem. 265, 18012-18022].
637-642
Divecha, N.
5c2ad0f8-4ce7-405f-8a15-2fc4ab96d787
Brooksbank, C. E.
45b02b1b-af77-453f-bdae-97cb8e3762ff
Irvine, R. F.
a8a94b1b-419c-4262-b745-eae1941ce145
1992
Divecha, N.
5c2ad0f8-4ce7-405f-8a15-2fc4ab96d787
Brooksbank, C. E.
45b02b1b-af77-453f-bdae-97cb8e3762ff
Irvine, R. F.
a8a94b1b-419c-4262-b745-eae1941ce145
Divecha, N., Brooksbank, C. E. and Irvine, R. F.
(1992)
Purification and characterization of phosphatidylinositol 4-phosphate 5-kinases.
Biochemical Journal, 288 (2), .
(doi:10.1042/bj2880637).
Abstract
We detail the purification and characterization of three distinct isoforms of PtdIns4P 5-kinase present in bovine brain. One of these, PtdIns4P 5-kinase C, was purified to apparent homogeneity, and SDS/PAGE analysis demonstrated a single polypeptide and molecular mass 53 KDa. These three isoforms were shown to differ in their kinetic properties, and immunological characterization with an antibody raised to PtdIns4P 5-kinase C demonstrated that this isoform was unrelated to the other two. Furthermore, PtdIns4P 5-kinase C was shown to be the bovine brain homologue of the Type II PtdIns4P 5-kinase previously purified from human erythrocytes [Bazenet, Ruano, Brockman & Anderson (1990) J. Biol. Chem. 265, 18012-18022].
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Published date: 1992
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Local EPrints ID: 480119
URI: http://eprints.soton.ac.uk/id/eprint/480119
ISSN: 0264-6021
PURE UUID: e8ca8f67-7049-44b0-b9f4-dec9775b4964
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Date deposited: 01 Aug 2023 16:51
Last modified: 17 Mar 2024 02:59
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Author:
C. E. Brooksbank
Author:
R. F. Irvine
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