A new phosphoramidite enables orthogonal double labelling to form combination oligonucleotide probes
A new phosphoramidite enables orthogonal double labelling to form combination oligonucleotide probes
Oligonucleotides labelled with thiazole orange intercalator and a reporter dye on the same thymine base have been synthesized. The key phosphoramidite (AP-C3 dT) contains an alkyne and amine, enabling dual orthogonal labelling of the nucleobase. Multiple monomers can be added to produce heavily functionalised oligonucleotides. In their DNA and 2′-OMe RNA formats these combination probes display high duplex stability and fluorescence when bound to complementary DNA and RNA.
8618-8622
Bai, Chunsen
fd0d744c-6edb-4395-8793-376d571e9b43
Klimkowski, Piotr
19202fc2-2dbd-4486-b01b-e0a6574b3586
Jin, Cheng
a4632b5f-7030-44ba-b08c-bcfe4b002059
Kuchlyan, Jagannath
f879a8a9-6bcb-452d-8c29-44fe3a968dc0
El-Sagheer, Afaf
05b8295a-64ad-4fdf-ad57-c34934a46c04
Brown, Tom
a64aae36-bb30-42df-88a2-11be394e8c89
November 2022
Bai, Chunsen
fd0d744c-6edb-4395-8793-376d571e9b43
Klimkowski, Piotr
19202fc2-2dbd-4486-b01b-e0a6574b3586
Jin, Cheng
a4632b5f-7030-44ba-b08c-bcfe4b002059
Kuchlyan, Jagannath
f879a8a9-6bcb-452d-8c29-44fe3a968dc0
El-Sagheer, Afaf
05b8295a-64ad-4fdf-ad57-c34934a46c04
Brown, Tom
a64aae36-bb30-42df-88a2-11be394e8c89
Bai, Chunsen, Klimkowski, Piotr, Jin, Cheng, Kuchlyan, Jagannath, El-Sagheer, Afaf and Brown, Tom
(2022)
A new phosphoramidite enables orthogonal double labelling to form combination oligonucleotide probes.
Organic & Biomolecular Chemistry, 2022 (20), .
(doi:10.1039/D2OB01899C).
Abstract
Oligonucleotides labelled with thiazole orange intercalator and a reporter dye on the same thymine base have been synthesized. The key phosphoramidite (AP-C3 dT) contains an alkyne and amine, enabling dual orthogonal labelling of the nucleobase. Multiple monomers can be added to produce heavily functionalised oligonucleotides. In their DNA and 2′-OMe RNA formats these combination probes display high duplex stability and fluorescence when bound to complementary DNA and RNA.
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Accepted/In Press date: 24 October 2022
e-pub ahead of print date: 27 October 2022
Published date: November 2022
Identifiers
Local EPrints ID: 480420
URI: http://eprints.soton.ac.uk/id/eprint/480420
ISSN: 1477-0520
PURE UUID: 26612363-023e-4f6f-8dbb-1cfb7276474e
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Date deposited: 02 Aug 2023 16:34
Last modified: 17 Mar 2024 03:04
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Contributors
Author:
Chunsen Bai
Author:
Piotr Klimkowski
Author:
Cheng Jin
Author:
Jagannath Kuchlyan
Author:
Afaf El-Sagheer
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