Influence of iron-limited and excess continuous culture on the virulence properties of Neisseria gonorrhoeae
Influence of iron-limited and excess continuous culture on the virulence properties of Neisseria gonorrhoeae
Iron-limited growth of non-piliated gonococci resulted in the selection of 100% piliated bacteria. Iron-limited gonococci were extremely virulent in the guinea pig subcutaneous chamber model and inocula of even 12 bacteria grew rapidly and persisted. By contrast, cystine-limited (iron replete) gonococci retained piliation but did not survive in the chambers. Transition from iron- limited to glucose-limited growth resulted in a marked loss of piliation but the bacteria remained virulent. Additional proteins were detectable in sarkosyl-purified outer membranes of iron-limited gonococci but several proteins with molecular weights similar to those described in the literature for iron-restricted gonococci were detectable in cystine- or glucose-limited bacteria, urging caution when interpreting such data.
727–729
Keevil, C.W.
cb7de0a7-ce33-4cfa-af52-07f99e5650eb
Davies, D.B.
3774a46d-d278-4e5d-99d4-0ea046bf4172
Spillane, B.J.
705474fa-14b5-49af-b658-33235143b7d3
Mahenthiralingam, E.
d4b5826c-6b25-4e9c-b0b3-2626bc5b9039
1988
Keevil, C.W.
cb7de0a7-ce33-4cfa-af52-07f99e5650eb
Davies, D.B.
3774a46d-d278-4e5d-99d4-0ea046bf4172
Spillane, B.J.
705474fa-14b5-49af-b658-33235143b7d3
Mahenthiralingam, E.
d4b5826c-6b25-4e9c-b0b3-2626bc5b9039
Keevil, C.W., Davies, D.B., Spillane, B.J. and Mahenthiralingam, E.
(1988)
Influence of iron-limited and excess continuous culture on the virulence properties of Neisseria gonorrhoeae.
In,
Poolman, J.T.
(ed.)
Gonococci and Meningococci: Epidemiology, Genetics, Immunochemistry and Pathogenesis.
Dordrecht.
Springer Dordrecht, .
(doi:10.1007/978-94-009-1383-7_114).
Record type:
Book Section
Abstract
Iron-limited growth of non-piliated gonococci resulted in the selection of 100% piliated bacteria. Iron-limited gonococci were extremely virulent in the guinea pig subcutaneous chamber model and inocula of even 12 bacteria grew rapidly and persisted. By contrast, cystine-limited (iron replete) gonococci retained piliation but did not survive in the chambers. Transition from iron- limited to glucose-limited growth resulted in a marked loss of piliation but the bacteria remained virulent. Additional proteins were detectable in sarkosyl-purified outer membranes of iron-limited gonococci but several proteins with molecular weights similar to those described in the literature for iron-restricted gonococci were detectable in cystine- or glucose-limited bacteria, urging caution when interpreting such data.
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Published date: 1988
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Local EPrints ID: 480532
URI: http://eprints.soton.ac.uk/id/eprint/480532
PURE UUID: a437a94f-03ee-4e71-9458-2dcb35376965
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Date deposited: 04 Aug 2023 16:34
Last modified: 18 Mar 2024 02:54
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Contributors
Author:
D.B. Davies
Author:
B.J. Spillane
Author:
E. Mahenthiralingam
Editor:
J.T. Poolman
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