READ ME File For 'Drop-Seq RData and TB88 FCS Files' Dataset DOI: 10.5258/SOTON/D0933 ReadMe Author: Thomas James Bailey, University of Southampton This dataset supports the thesis entitled 'Role of Akt Signalling in CD8 T Cell Responses' AWARDED BY: University of Southampton DATE OF AWARD:2019 DESCRIPTION OF THE DATA R Data Files contains the functional data file from which all the Drop-Seq data has been derived. It will require R studio as well as the Seurat software package to open and analyse. TB88 Naive FCS Files contains the FCS files from the experiment TB88 (which can be referenced in my lab book, held by the Al-Shamkhani Lab Group, Cancer Sciences), specifically the files relating to the naive baseline cells TB88 45hrs WT FCS Files contains the FCS files from the experiment TB88, specifically the WT based cell data from the 45hr time point post listeria infection. TB88 45hrs PDK FCS Files contains the FCS files from the experiment TB88, specifically the PDK based cell data from the 45hr time point post listeria infection. These FCS files can be viewed using FCS Express, FlowJo or the software found on the Canto FACS systems. This dataset contains: 4 zipped files - R Data Files, TB88 Naive FCS Files, TB88 45hrs WT FCS Files, TB88 45hrs PDK FCS Files Date of data collection: 14/02/2018 - 16/02/2018 Information about geographic location of data collection: University of Southampton, U.K. Licence:CC-BY Related projects/Funders: Date that the file was created: 02, 2018