Controlling the positive ion electrospray ionization of poly(ethylene glycols) when using ultra-high-performance supercritical fluid chromatography-mass spectrometry
Controlling the positive ion electrospray ionization of poly(ethylene glycols) when using ultra-high-performance supercritical fluid chromatography-mass spectrometry
Poly(ethylene glycols) are complex polymers often added to pharmaceutical formulations to improve drug solubility and delivery. One of the main challenges when using chromatographic techniques coupled to mass spectrometry is the unselective ionization of poly(ethylene glycols) oligomers. Additionally, when the chain length is large enough, multiple charged species are formed, further complicating the mass spectra and processing. This study uses the advanced oligomer separation provided by supercritical fluid chromatography with a mass spectrometry approach that selectively ionizes poly(ethylene glycols) as ammoniated molecules to simplify data analysis and facilitate batch-to-batch comparisons. Several visual representations of the response of the ionization events based on the polymer molecular weight and the repeating unit were used to elucidate trends in ionization. Evaluation of the influence of the oligomer length and end-group on the electrospray ionization of the polymer allowed the development of a process to enable selective ionization for these complex polymers.
electrospray ionization, mass spectrometry, poly(ethylene glycol), polymers, supercritical fluid chromatography
Cancho Gonzalez, Sergio
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Ferguson, Paul
99fa9381-bc43-4985-9ee7-e295eb6c49cf
Herniman, Julie M.
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Langley, G. John
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October 2023
Cancho Gonzalez, Sergio
1fc211b0-5c0f-4435-8935-51eef7f2f212
Ferguson, Paul
99fa9381-bc43-4985-9ee7-e295eb6c49cf
Herniman, Julie M.
530b1a36-1386-4602-8df7-defa6eb3512b
Langley, G. John
7ac80d61-b91d-4261-ad17-255f94ea21ea
Cancho Gonzalez, Sergio, Ferguson, Paul, Herniman, Julie M. and Langley, G. John
(2023)
Controlling the positive ion electrospray ionization of poly(ethylene glycols) when using ultra-high-performance supercritical fluid chromatography-mass spectrometry.
Journal of Separation Science, 46 (20), [2300425].
(doi:10.1002/jssc.202300425).
Abstract
Poly(ethylene glycols) are complex polymers often added to pharmaceutical formulations to improve drug solubility and delivery. One of the main challenges when using chromatographic techniques coupled to mass spectrometry is the unselective ionization of poly(ethylene glycols) oligomers. Additionally, when the chain length is large enough, multiple charged species are formed, further complicating the mass spectra and processing. This study uses the advanced oligomer separation provided by supercritical fluid chromatography with a mass spectrometry approach that selectively ionizes poly(ethylene glycols) as ammoniated molecules to simplify data analysis and facilitate batch-to-batch comparisons. Several visual representations of the response of the ionization events based on the polymer molecular weight and the repeating unit were used to elucidate trends in ionization. Evaluation of the influence of the oligomer length and end-group on the electrospray ionization of the polymer allowed the development of a process to enable selective ionization for these complex polymers.
Text
2023_08_08_SCG paper selective ionisation and SFC v10
- Accepted Manuscript
Text
J of Separation Science - 2023 - Cancho‐Gonzalez
- Version of Record
More information
Accepted/In Press date: 9 August 2023
Published date: October 2023
Additional Information:
Funding Information:
The authors would like to acknowledge the valuable discussions with Dr. Matthias Baud. The authors appreciate the financial support provided by AstraZeneca and the UK Engineering and Physical Sciences Research Council to support this research project.
Publisher Copyright:
© 2023 The Authors. Journal of Separation Science published by Wiley-VCH GmbH.
Keywords:
electrospray ionization, mass spectrometry, poly(ethylene glycol), polymers, supercritical fluid chromatography
Identifiers
Local EPrints ID: 481346
URI: http://eprints.soton.ac.uk/id/eprint/481346
ISSN: 1615-9314
PURE UUID: 98242891-1c3f-4902-8f20-47b8900d103d
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Date deposited: 23 Aug 2023 17:02
Last modified: 18 Mar 2024 02:50
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Author:
Sergio Cancho Gonzalez
Author:
Paul Ferguson
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