READ ME File For 'Dataset supporting the University of Southampton Doctoral Thesis "Antibody Engineering for improving Breast Cancer Therapeutics"'

Dataset DOI: 10.5258/SOTON/D2637

ReadMe Author: HANNAH SMITH, University of Southampton ORCID ID: https://orcid.org/0000-0002-7064-5037

This dataset supports the thesis entitled Antibody Engineering for improving Breast Cancer Therapeutics
AWARDED BY: Univeristy of Southampton
DATE OF AWARD: 2023

DESCRIPTION OF THE DATA
All data in this dataset relates to single and tandem trastuzumab-based antibodies generated as part of Hannah Smith's PhD project. this dataset includes:
- Negtiave electron microscopy images which were collected by Sonya James. She holds the raw files are are not included in this dataset, only the exported files are included in this dataset.
- Glycan analysis data of the Fc glycan sites which was collected by Joel Allen. Raw data in Microsoft excel files. 
- Surface plasmon resonance data of antibody-Fc or antibody-antigen interactions collected via Biacore. Raw data collected from the Biacore evauation software and exported as a .txt file.
- Raw luminescience data from cytotoxicity assays was collected on a Varioskan plate reader and raw exported in Microsft excel files. 
- Flow cytometry data from phagocytosis assays or measurments of antigen expression collected on the BD FACs canto. Raw .fcs files require FlowJo or FCS express to be veiwed. 
- All data analysis was completed using GraphPad Prism

This dataset contains:

Murine_SPR_Data.zip
Human_SPR_Data.zip
ADCC_Data_for Pure.zip
MCF7 ADCP Data_for_Pure.zip
MDAMB-453_ADCP_Data_for_Pure.zip
Glycan_Analysis_Data_for_Pure.zip
Negative_EM_Data_for_Pure.zip


Date of data collection: 2018-2022

Licence:Creative Commons Attribution CC-BY 

Related projects/Funders:
Against Breast Cancer, Grant code:518369101