ReadMe Author: KATIE HORTON, University of Southampton ORCID ID 0000-0002-9357-928X

This dataset supports the thesis entitled Towards Understanding the Susceptibility of Primary Ciliary Dyskinesia Airway Epithelia to Non-Typeable Haemophilus influenzae Biofilm Infection
AWARDED BY: Univeristy of Southampton
DATE OF AWARD: 2023

DESCRIPTION OF THE DATA
All data collected as part of this thesis are held by the Primary Ciliary Dyskinesia Group on the University of Southampton J: drive and will be available upon request after the embargo period ending on 01/01/2025. Data can be requested by verified academic staff by emailing the Principal Investigator Professor Jane Lucas jlucas1@soton.ac.uk. Characterisation of the air-liquid-interface-culture model data includes raw transepithelial electrical resistance measurements, confocal microscopy images, videos of ciliary beating, scanning electron micrographs and raw colony forming units. 

The genome of a primary ciliary dyskinesia isolate of non-typeable Haemophilus influenzae was sequenced and data are genbank and FASTA files. 

Flow cytometry was performed to understand non-typeable Haemophilus influenzae internalisation and data are FACS plots. 

Liquid chromatography mass spectrometry was done on nasal epithelial cells from donors with and without primary ciliary dyskinesia, and with and without non-typeable Haemophilus influenzae infection. Data are .csv file PEAKS outputs. 

Date of data collection: 10,2018 - 7,2023

Date that the file was created: 08, 2023