READ ME File For: Dataset in support of the article 'Characterising reef net metabolism via the diel co-variation of pH and dissolved oxygen from high resolution in situ sensors.' Dataset DOI: 10.5258/SOTON/D2346 Date that the file was created: August, 2023 ------------------- GENERAL INFORMATION ------------------- ReadMe Author: Sarah Cryer, University of Southampton [0000-0001-7192-7426] Date of data collection: 25 October 2019 - 04 Novemeber 2019 (Goff's Caye, Belize) 06 November 2019 - 23 December 2019 (Engish Caye, Belize) 15 March 2018 - 29 March 2018 (Soufrière, Dominica) 9 April 2018 – 20 April 2018 (Soufrière, Dominica) 08 June 2018 – 24 June 2018 (Soufrière, Dominica) 27 March 2019 – 07 April 2019 (Soufrière, Dominica) 03 July 2019 – 14 July 2019 (Soufrière, Dominica) Information about geographic location of data collection: Goff’s Caye Fore Reef, Belize: 17.35229°N, 88.03381°W Goff’s Caye Back Reef, Belize: 17.35401°N, 88.03789°W English Caye, Belize: 17.326094°N, 88.04933°W Soufrière, Dominica: 15.236866°N, 61.370282°W Related projects: Commonwealth Marine Economies Programme Belize & Dominica. -------------------------- SHARING/ACCESS INFORMATION -------------------------- Licenses/restrictions placed on the data, or limitations of reuse: na Recommended citation for the data: Cryer et al., 2023 This dataset supports the publication: AUTHORS: Sarah E Cryer, Claire Evans, Sara E. Fowell, Gilbert Andrews, Peter Brown, Filipa Carvalho, Diana Degallerie, Jake Ludgate, Samir Rosado, Richard Sanders, James A. Strong, Derrick Theophille, Arlene Young, and Socratis Loucaides TITLE: Characterising reef net metabolism via the diel co-variation of pH and dissolved oxygen from high resolution in situ sensors. JOURNAL: Global Biogeochemical Cycles PAPER DOI: 10.1029/2022GB007577 Links to other publicly accessible locations of the data: Links/relationships to ancillary or related data sets: Project PI: Dr Socratis Loucaides, National Oceanography Centre Funded By: Foreign & Commonwealth Office: Commonwealth Marine Economies Progamme. -------------------- DATA & FILE OVERVIEW -------------------- This dataset contains: [File list (filenames, directory structure (for zipped files) and brief description of all data files)] There are six csv and 1 excel files associated, each tab of the excel file is one of the csv files Cryer_et_al_pH_DO_Data_for_repository - Includes metadata tab explaining headers and data collection for the following datasets. Each tab is pH, DO, salinity and temperature senesor data from each site (Goff's Caye Fore Reef, Belize; Goff's Caye Back Reef, Belize; English Caye, BelizeSoufrière, Dominica). Goff's Caye sites additionally have Total Alkalinity and Dissolved Inorganic Carbon Data. Cryer_et_al_pH_DO_Caribbean_Metadata - Metadata file explaining headers and data collection for the following dataset files. Cryer_et_al_pH_DO_Caribbean_GoffsCayeForeReef - pH, DO, salinity and temperature data collected on the fore reef of Goff's Caye, Belize. Additionally there is a total alkalinity and dissolved inorganic carbon measurement from a discrete sample. Cryer_et_al_pH_DO_Caribbean_GoffsCayeBackReef - pH, DO, salinity and temperature data collected on the back reef of Goff's Caye, Belize using sensors above. Additionally there is a total alkalinity and dissolved inorganic carbon measurement from a discrete sample. Cryer_et_al_pH_DO_Caribbean_EnglishCaye - pH, DO, salinity and temperature data collected on the back reef of English Caye, Belize using sensors above. Cryer_et_al_pH_DO_Caribbean_Dominica - pH, DO, salinity and temperature data collected on the back reef near Soufriere, Dominica using sensors above. Cryer_et_al_pH_DO_Caribbean_Benthos - Benthic composition collected from photographs of reef sites using Coral Point Count. Relationship between files, if important for context: Excel file is all the csv files combined into one file, each csv file is a tab. Additional related data collected that was not included in the current data package: If data was derived from another source, list source: If there are there multiple versions of the dataset, list the file updated, when and why update was made: -------------------------- METHODOLOGICAL INFORMATION -------------------------- Description of methods used for collection/generation of data: Data were collected to obtain high-resolution pH and dissolved oxygen measurements on four different sites in the Caribbean. Three sites on the Mesoamerican Reef in Belize and a single site in Dominica. Each platform includes a microfluidic lab-on-chip (LOC) pH sensor (Yin et al., 2021) and a pumped conductivity, temperature and dissolved oxygen (CT-DO) sensor (SBE 37 SMP ODO MicroCAT; Seabird Scientific, Bellevue WA, USA). Power was provided by a custom-made rechargeable Ni-MH battery pack enclosed within a PVC 100 m depth rated housing. Benthic ecosystem assemblages around each observatory were characterized from seabed photographs taken using high resolution underwater cameras. In Belize, photos were taken using a GoPro Hero 3+ by scuba divers or snorkelers immediately after platform deployment. In Dominica, a GoPro mounted on a mini Remotely Operated Vehicle (DeepTrecker) was deployed over the side of the boat and screengrabs were taken from recorded video. Dominica photos were taken on 10/03/2019 (1-2 years post deployment). Benthos and substrate were determined using Coral Point Count with Excel Extensions (Kohler and Gill, 2006). Four photos were loaded for each of the Belize sites and 13 screengrabs for Dominica. For each photo an 8 x 8 grid was constructed and within each cell, a point was randomly distributed. The marine benthos or substratum for each point was then identified manually under 11 categories as specified in the benthos csv file. References Kohler, K.E., Gill, S.M., 2006. Coral Point Count with Excel extensions (CPCe): A Visual Basic program for the determination of coral and substrate coverage using random point count methodology. Comput. Geosci. 32, 1259–1269. Yin, T., Papadimitriou, S., Rérolle, V.M.C., Arundell, M., Cardwell, C.L., Walk, J., Palmer, M.R., Fowell, S.E., Schaap, A., Mowlem, M.C., Loucaides, S., 2021. A Novel Lab-on-Chip Spectrophotometric pH Sensor for Autonomous In Situ Seawater Measurements to 6000 m Depth on Stationary and Moving Observing Platforms . Environ. Sci. Technol. 55, 14968–14978. Methods for processing the data: Sensor data was used before biofouling became evident from the pH data - biofouling created large amplitudes in pH. Software- or Instrument-specific information needed to interpret the data, including software and hardware version numbers: n/a Standards and calibration information, if appropriate: Total Alkalinity TA was determined using a two-stage potentiometric open-cell titration using a Scripps Total Alkalinity Titration System (Dickson et al., 2003). The titration acid was calibrated each day using CRMs (Certified Reference Material, Dickson Lab Batches 180 and 191; Dickson, 2010), with an average uncertainty on CRMs of ±1.7 µmol/kg. Dissolved Inorganic Carbon was measured by coulometry using a MARIANDA VINDTA 3C system (Mintrop, 2004). CRMs (Batches 180 and 191; Dickson, 2010) were run every ~12 samples, with an average uncertainty of ±3.0 µmol/kg Environmental/experimental conditions: Describe any quality-assurance procedures performed on the data: People involved with sample collection, processing, analysis and/or submission: Sarah Cryer, Socratis Loucaides, Sara Fowell, Urska Martinicic, Samir Rosado, Gilbert Andrews, Arlene Young, Diana Degallerie, Derrick Theophille -------------------------- DATA-SPECIFIC INFORMATION -------------------------- Number of variables: 11 Number of cases/rows: 234 Variable list, defining any abbreviations, units of measure, codes or symbols used: DateTime: Local Time (Belize) Depth: metres Salinity: g/kg O2: mg/L O2_umol: umol/L Total Alkalinity: umol/kg Dissolved Inorganic Carbon: umol/kg Missing data codes: NaN Specialized formats or other abbreviations used: -------------------------- DATA-SPECIFIC INFORMATION -------------------------- Number of variables: 11 Number of cases/rows: 234 Variable list, defining any abbreviations, units of measure, codes or symbols used: DateTime: Local Time (Belize) Depth: metres Salinity: g/kg O2: ml/L O2_umol: umol/L Total Alkalinity: umol/kg Dissolved Inorganic Carbon: umol/kg Missing data codes: NaN Specialized formats or other abbreviations used: -------------------------- DATA-SPECIFIC INFORMATION -------------------------- Number of variables: 9 Number of cases/rows: 558 Variable list, defining any abbreviations, units of measure, codes or symbols used: DateTime: Local Time (Belize) Depth: metres Salinity: g/kg O2: ml/L O2_umol: umol/L Total Alkalinity: umol/kg Dissolved Inorganic Carbon: umol/kg Missing data codes: NaN Specialized formats or other abbreviations used: -------------------------- DATA-SPECIFIC INFORMATION -------------------------- Number of variables: 9 Number of cases/rows: 739 Variable list, defining any abbreviations, units of measure, codes or symbols used: DateTime: Local Time (Belize) Depth: metres Salinity: g/kg O2: ml/L O2_umol: umol/L Missing data codes: NaN Specialized formats or other abbreviations used: -------------------------- DATA-SPECIFIC INFORMATION -------------------------- Number of variables: 9 Number of cases/rows: 739 Variable list, defining any abbreviations, units of measure, codes or symbols used: DateTime: Local Time (Belize) Depth: metres Salinity: g/kg O2: ml/L O2_umol: umol/L Missing data codes: NaN Specialized formats or other abbreviations used: