In situ SERS probing of nano-silver coated individual yeast cells
In situ SERS probing of nano-silver coated individual yeast cells
For understanding cells functionalities and their communications, there is a need for highly sensitive cell analysis platforms capable of assessing non-specific chemicals on the surface and in the vicinity of cells. We report a microfluidic system integrating dielectrophoresis and surface enhanced Raman scattering (SERS) for the trapping and real time monitoring of cell functions in isolated and grouped cell clusters. Yeast cells are coated with silver nanoparticles to enable highly sensitive SERS analysis. The SERS responses of cells are examined under various conditions: live vs. dead and isolated vs. grouped. This work illustrates the feasibility of the system for in situ cell monitoring and analysis of secreted chemicals during their growth, metabolism, proliferation and apoptosis.
Dielectrophoresis, Microfluidics, Principal component analysis, SERS, Yeast cell
536-541
Chrimes, Adam F.
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Khoshmanesh, Khashayar
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Tang, Shi Yang
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Wood, Bayden R.
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Stoddart, Paul R.
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Collins, Sean S.E.
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Mitchell, Arnan
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Kalantar-zadeh, Kourosh
aded6a64-8612-40b7-aae9-233fbae916a6
15 November 2013
Chrimes, Adam F.
67cd9f67-6f5b-4682-bcd1-2b4692ede9ae
Khoshmanesh, Khashayar
72608a1e-0e41-4360-8065-98d872493aed
Tang, Shi Yang
1d0f15c6-2a3e-4bad-a3d8-fc267db93ed4
Wood, Bayden R.
edad4051-2e75-4067-8d4a-ab530b298f93
Stoddart, Paul R.
7ff1803c-680c-45a6-8b82-8b1486c29b59
Collins, Sean S.E.
0fe01801-239d-405a-b824-280e730668df
Mitchell, Arnan
33a18604-17e3-421e-b895-0f420d332cd4
Kalantar-zadeh, Kourosh
aded6a64-8612-40b7-aae9-233fbae916a6
Chrimes, Adam F., Khoshmanesh, Khashayar, Tang, Shi Yang, Wood, Bayden R., Stoddart, Paul R., Collins, Sean S.E., Mitchell, Arnan and Kalantar-zadeh, Kourosh
(2013)
In situ SERS probing of nano-silver coated individual yeast cells.
Biosensors and Bioelectronics, 49, .
(doi:10.1016/j.bios.2013.05.053).
Abstract
For understanding cells functionalities and their communications, there is a need for highly sensitive cell analysis platforms capable of assessing non-specific chemicals on the surface and in the vicinity of cells. We report a microfluidic system integrating dielectrophoresis and surface enhanced Raman scattering (SERS) for the trapping and real time monitoring of cell functions in isolated and grouped cell clusters. Yeast cells are coated with silver nanoparticles to enable highly sensitive SERS analysis. The SERS responses of cells are examined under various conditions: live vs. dead and isolated vs. grouped. This work illustrates the feasibility of the system for in situ cell monitoring and analysis of secreted chemicals during their growth, metabolism, proliferation and apoptosis.
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Published date: 15 November 2013
Additional Information:
Funding Information:
Dr. Khoshmanesh is the recipient of the Discovery Early Career Researcher Award (DECRA) under project DE120101402 . Dr. Wood is supported by an ARC QEII Fellowship grant, DP0878464 . The authors acknowledge the facilities, and the scientific and technical assistance, of the Australian Microscopy & Microanalysis Research Facility at RMIT University.
Keywords:
Dielectrophoresis, Microfluidics, Principal component analysis, SERS, Yeast cell
Identifiers
Local EPrints ID: 481654
URI: http://eprints.soton.ac.uk/id/eprint/481654
ISSN: 0956-5663
PURE UUID: 76d8227d-7bcf-491d-9f39-8ddc59e1bae9
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Date deposited: 05 Sep 2023 16:56
Last modified: 18 Mar 2024 04:13
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Contributors
Author:
Adam F. Chrimes
Author:
Khashayar Khoshmanesh
Author:
Shi Yang Tang
Author:
Bayden R. Wood
Author:
Paul R. Stoddart
Author:
Sean S.E. Collins
Author:
Arnan Mitchell
Author:
Kourosh Kalantar-zadeh
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