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Modifying dielectrophoretic response of nonviable yeast cells by ionic surfactant treatment

Modifying dielectrophoretic response of nonviable yeast cells by ionic surfactant treatment
Modifying dielectrophoretic response of nonviable yeast cells by ionic surfactant treatment

Nonviable cells are essential biosystems, due to the functionalities they offer and their effects on viable cells. Therefore, the separation and immobilization of nonviable cells separately or in the vicinity of viable cells is of great importance for many fundamentals investigations in cell biology. However, most nonviable cells become less polarizable than the surrounding medium at conductivities above 0.01 S/m. This means that in such a medium, dielectrophoresis, despite its great versatilities for manipulation of cells, cannot be employed for immobilizing nonviable cells. Here, we present a novel approach to change the dielectrophoretic (DEP) response of nonviable yeast cells by treating them with low concentrations of ionic surfactants such as sodium dodecyl sulfate. After this treatment, they exhibit a strong positive DEP response, even at high medium conductivities. The capability of this treatment is demonstrated in two proof-of-concept experiments. First, we show the sorting and immobilization of viable and nonviable yeast cells, along consecutive microelectrode arrays. Second, we demonstrate the immobilization of viable and nonviable cells in the vicinity of each other along the same microelectrode array. The proposed technique allows DEP platforms to be utilized for the immobilization and subsequent postanalysis of both viable and nonviable cells with and without the presence of each other.

0003-2700
6364-6371
Tang, Shi Yang
1d0f15c6-2a3e-4bad-a3d8-fc267db93ed4
Zhang, Wei
580a3151-098d-4fac-b791-931fa89fa327
Baratchi, Sara
aec99b88-f6af-499d-b71d-2cfbc5fab461
Nasabi, Mahyar
5805bfab-fc16-4873-bc03-d49ffbd8dd19
Kalantar-Zadeh, Kourosh
aded6a64-8612-40b7-aae9-233fbae916a6
Khoshmanesh, Khashayar
72608a1e-0e41-4360-8065-98d872493aed
Tang, Shi Yang
1d0f15c6-2a3e-4bad-a3d8-fc267db93ed4
Zhang, Wei
580a3151-098d-4fac-b791-931fa89fa327
Baratchi, Sara
aec99b88-f6af-499d-b71d-2cfbc5fab461
Nasabi, Mahyar
5805bfab-fc16-4873-bc03-d49ffbd8dd19
Kalantar-Zadeh, Kourosh
aded6a64-8612-40b7-aae9-233fbae916a6
Khoshmanesh, Khashayar
72608a1e-0e41-4360-8065-98d872493aed

Tang, Shi Yang, Zhang, Wei, Baratchi, Sara, Nasabi, Mahyar, Kalantar-Zadeh, Kourosh and Khoshmanesh, Khashayar (2013) Modifying dielectrophoretic response of nonviable yeast cells by ionic surfactant treatment. Analytical Chemistry, 85 (13), 6364-6371. (doi:10.1021/ac400741v).

Record type: Article

Abstract

Nonviable cells are essential biosystems, due to the functionalities they offer and their effects on viable cells. Therefore, the separation and immobilization of nonviable cells separately or in the vicinity of viable cells is of great importance for many fundamentals investigations in cell biology. However, most nonviable cells become less polarizable than the surrounding medium at conductivities above 0.01 S/m. This means that in such a medium, dielectrophoresis, despite its great versatilities for manipulation of cells, cannot be employed for immobilizing nonviable cells. Here, we present a novel approach to change the dielectrophoretic (DEP) response of nonviable yeast cells by treating them with low concentrations of ionic surfactants such as sodium dodecyl sulfate. After this treatment, they exhibit a strong positive DEP response, even at high medium conductivities. The capability of this treatment is demonstrated in two proof-of-concept experiments. First, we show the sorting and immobilization of viable and nonviable yeast cells, along consecutive microelectrode arrays. Second, we demonstrate the immobilization of viable and nonviable cells in the vicinity of each other along the same microelectrode array. The proposed technique allows DEP platforms to be utilized for the immobilization and subsequent postanalysis of both viable and nonviable cells with and without the presence of each other.

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More information

Published date: 2 July 2013

Identifiers

Local EPrints ID: 481655
URI: http://eprints.soton.ac.uk/id/eprint/481655
ISSN: 0003-2700
PURE UUID: 0c97e54b-522a-409b-af0f-6f16dbc09670
ORCID for Shi Yang Tang: ORCID iD orcid.org/0000-0002-3079-8880

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Date deposited: 05 Sep 2023 16:56
Last modified: 18 Mar 2024 04:13

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Contributors

Author: Shi Yang Tang ORCID iD
Author: Wei Zhang
Author: Sara Baratchi
Author: Mahyar Nasabi
Author: Kourosh Kalantar-Zadeh
Author: Khashayar Khoshmanesh

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