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Reorientation of microfluidic channel enables versatile dielectrophoretic platforms for cell manipulations

Reorientation of microfluidic channel enables versatile dielectrophoretic platforms for cell manipulations
Reorientation of microfluidic channel enables versatile dielectrophoretic platforms for cell manipulations

Dielectrophoresis is a versatile tool for the sorting, immobilization, and characterization of cells in microfluidic systems. The performance of dielectrophoretic systems strongly relies on the configuration of microelectrodes, which produce a nonuniform electric field. However, once fabricated, the microelectrodes cannot be reconfigured to change the characteristics of the system. Here, we show that the reorientation of the microfluidic channel with respect to the microelectrodes can be readily utilized to alter the characteristics of the system. This enables us to change the location and density of immobilized viable cells across the channel, release viable cells along customized numbers of streams within the channel, change the deflection pattern of nonviable cells along the channel, and improve the sorting of viable and nonviable cells in terms of flow throughput and efficiency of the system. We demonstrate that the reorientation of the microfluidic channel is an effective tool to create versatile dielectrophoretic platforms using the same microelectrode design.

Dielectrophoresis, Microfluidics, Sorting, Trapping, Yeast
0173-0835
1407-1414
Tang, Shi Yang
1d0f15c6-2a3e-4bad-a3d8-fc267db93ed4
Zhang, Wei
580a3151-098d-4fac-b791-931fa89fa327
Yi, Pyshar
497e4f29-d085-4011-b8fa-19d515591f6d
Baratchi, Sara
aec99b88-f6af-499d-b71d-2cfbc5fab461
Kalantar-zadeh, Kourosh
aded6a64-8612-40b7-aae9-233fbae916a6
Khoshmanesh, Khashayar
72608a1e-0e41-4360-8065-98d872493aed
Tang, Shi Yang
1d0f15c6-2a3e-4bad-a3d8-fc267db93ed4
Zhang, Wei
580a3151-098d-4fac-b791-931fa89fa327
Yi, Pyshar
497e4f29-d085-4011-b8fa-19d515591f6d
Baratchi, Sara
aec99b88-f6af-499d-b71d-2cfbc5fab461
Kalantar-zadeh, Kourosh
aded6a64-8612-40b7-aae9-233fbae916a6
Khoshmanesh, Khashayar
72608a1e-0e41-4360-8065-98d872493aed

Tang, Shi Yang, Zhang, Wei, Yi, Pyshar, Baratchi, Sara, Kalantar-zadeh, Kourosh and Khoshmanesh, Khashayar (2013) Reorientation of microfluidic channel enables versatile dielectrophoretic platforms for cell manipulations. Electrophoresis, 34 (9-10), 1407-1414. (doi:10.1002/elps.201200659).

Record type: Article

Abstract

Dielectrophoresis is a versatile tool for the sorting, immobilization, and characterization of cells in microfluidic systems. The performance of dielectrophoretic systems strongly relies on the configuration of microelectrodes, which produce a nonuniform electric field. However, once fabricated, the microelectrodes cannot be reconfigured to change the characteristics of the system. Here, we show that the reorientation of the microfluidic channel with respect to the microelectrodes can be readily utilized to alter the characteristics of the system. This enables us to change the location and density of immobilized viable cells across the channel, release viable cells along customized numbers of streams within the channel, change the deflection pattern of nonviable cells along the channel, and improve the sorting of viable and nonviable cells in terms of flow throughput and efficiency of the system. We demonstrate that the reorientation of the microfluidic channel is an effective tool to create versatile dielectrophoretic platforms using the same microelectrode design.

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More information

Published date: May 2013
Keywords: Dielectrophoresis, Microfluidics, Sorting, Trapping, Yeast

Identifiers

Local EPrints ID: 481657
URI: http://eprints.soton.ac.uk/id/eprint/481657
ISSN: 0173-0835
PURE UUID: 29f4eea2-42d7-461d-92e7-cbc6c5ab739e
ORCID for Shi Yang Tang: ORCID iD orcid.org/0000-0002-3079-8880

Catalogue record

Date deposited: 05 Sep 2023 16:56
Last modified: 18 Mar 2024 04:13

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Contributors

Author: Shi Yang Tang ORCID iD
Author: Wei Zhang
Author: Pyshar Yi
Author: Sara Baratchi
Author: Kourosh Kalantar-zadeh
Author: Khashayar Khoshmanesh

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