Discontinuous dielectrophoresis a technique for investigating the response of loosely adherent cells to high shear stress
Discontinuous dielectrophoresis a technique for investigating the response of loosely adherent cells to high shear stress
The functioning of cells under mechanical stress influences several cellular processes, for example proliferation, organogenesis, and transcription. Current techniques used to examine mechanical stress on loosely adherent cells, are however, primarily focused on single individual cells being stimulated, or require time-consuming surface coating techniques; and are limited in the level of shear stress that can be supplied to immobilised cells. Here we report the process of the technique, discontinuous dielectrophoresis; which enables high shear stress analysis of clusters of immobilised loosely adherent cells, we have analysed the performance of the system using Saccharomyces cerevisiae yeast cells, up to a shear stress of 42 dyn/cm2. Additionally, we provide application experimental results from investigating shear induced calcium signalling of HEK-293-TRPV4 cells at flow rates of 2.5, and 120 μl/min, corresponding to shear stress levels of 0.875 and 42 dyn/cm2, respectively. In summary, discontinuous dielectrophoresis will enable the investigation of the mechanotransduction behaviour of loosely adherent cells under physiologically relevant shear stresses. Additionally, discontinuous dielectrophoresis provides the capability for parallelism, and dynamic control over the microenvironment, as previously explored by different microfluidic platforms without the capacity for high shear stress analysis of loosely adherent cells.
Dielectrophoresis, Discontinuous dielectrophoresis, HEK-293, Intracellular calcium signalling, Microfluidics, Shear-induced stress
23-33
Soffe, Rebecca
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Baratchi, Sara
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Tang, Shi Yang
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Mcintyre, Peter
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Mitchell, Arnan
33a18604-17e3-421e-b895-0f420d332cd4
Khoshmanesh, Khashayar
72608a1e-0e41-4360-8065-98d872493aed
2016
Soffe, Rebecca
c1670df9-4ac4-4abc-8c48-3f6048a4e9fb
Baratchi, Sara
aec99b88-f6af-499d-b71d-2cfbc5fab461
Tang, Shi Yang
1d0f15c6-2a3e-4bad-a3d8-fc267db93ed4
Mcintyre, Peter
c05d25d3-e22e-4561-a0c5-c6bb14c24955
Mitchell, Arnan
33a18604-17e3-421e-b895-0f420d332cd4
Khoshmanesh, Khashayar
72608a1e-0e41-4360-8065-98d872493aed
Soffe, Rebecca, Baratchi, Sara, Tang, Shi Yang, Mcintyre, Peter, Mitchell, Arnan and Khoshmanesh, Khashayar
(2016)
Discontinuous dielectrophoresis a technique for investigating the response of loosely adherent cells to high shear stress.
Gilbert, James, Azhari, Haim, Ali, Hesham, Quintao, Carla, Sliwa, Jan, Ruiz, Carolina, Fred, Ana and Gamboa, Hugo
(eds.)
In BIODEVICES 2016 - 9th International Conference on Biomedical Electronics and Devices, Proceedings; Part of 9th International Joint Conference on Biomedical Engineering Systems and Technologies, BIOSTEC 2016.
SciTePress.
.
(doi:10.5220/0005654700230033).
Record type:
Conference or Workshop Item
(Paper)
Abstract
The functioning of cells under mechanical stress influences several cellular processes, for example proliferation, organogenesis, and transcription. Current techniques used to examine mechanical stress on loosely adherent cells, are however, primarily focused on single individual cells being stimulated, or require time-consuming surface coating techniques; and are limited in the level of shear stress that can be supplied to immobilised cells. Here we report the process of the technique, discontinuous dielectrophoresis; which enables high shear stress analysis of clusters of immobilised loosely adherent cells, we have analysed the performance of the system using Saccharomyces cerevisiae yeast cells, up to a shear stress of 42 dyn/cm2. Additionally, we provide application experimental results from investigating shear induced calcium signalling of HEK-293-TRPV4 cells at flow rates of 2.5, and 120 μl/min, corresponding to shear stress levels of 0.875 and 42 dyn/cm2, respectively. In summary, discontinuous dielectrophoresis will enable the investigation of the mechanotransduction behaviour of loosely adherent cells under physiologically relevant shear stresses. Additionally, discontinuous dielectrophoresis provides the capability for parallelism, and dynamic control over the microenvironment, as previously explored by different microfluidic platforms without the capacity for high shear stress analysis of loosely adherent cells.
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More information
Published date: 2016
Additional Information:
Funding Information:
Khashayar Khoshmanesh acknowledges the Australian Research Council for funding, under the Discovery Early Career Researcher Award (DECRA) scheme, (project DE120101402).
Publisher Copyright:
© 2016 by SCITEPRESS - Science and Technology Publications, Lda. All rights reserved.
Venue - Dates:
9th International Conference on Biomedical Electronics and Devices, BIODEVICES 2016 - Part of 9th International Joint Conference on Biomedical Engineering Systems and Technologies, BIOSTEC 2016, , Rome, Italy, 2016-02-21 - 2016-02-23
Keywords:
Dielectrophoresis, Discontinuous dielectrophoresis, HEK-293, Intracellular calcium signalling, Microfluidics, Shear-induced stress
Identifiers
Local EPrints ID: 481680
URI: http://eprints.soton.ac.uk/id/eprint/481680
PURE UUID: 1549dd42-f21e-42c4-b9cb-978ee47ea4da
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Date deposited: 06 Sep 2023 16:48
Last modified: 18 Mar 2024 04:13
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Contributors
Author:
Rebecca Soffe
Author:
Sara Baratchi
Author:
Shi Yang Tang
Author:
Peter Mcintyre
Author:
Arnan Mitchell
Author:
Khashayar Khoshmanesh
Editor:
James Gilbert
Editor:
Haim Azhari
Editor:
Hesham Ali
Editor:
Carla Quintao
Editor:
Jan Sliwa
Editor:
Carolina Ruiz
Editor:
Ana Fred
Editor:
Hugo Gamboa
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