A portable, hand-powered microfluidic device for sorting of biological particles
A portable, hand-powered microfluidic device for sorting of biological particles
Manually hand-powered portable microfluidic devices are cheap alternatives for point-of-care diagnostics. Currently, on-field tests are limited by the use of bulky syringe pumps, pressure controller and equipment. In this work, we present a manually operated microfluidic device incorporated with a groove-based channel. We show that the device is capable to effectively sort particles/cells by manual hand powering. First, the grooved-based channel with differently sized polystyrene particles was characterized using syringe pumps to study their distributions under various flow rate conditions. Afterward, the particle mixtures were sorted manually using hand power to verify the capability of this device. Finally, the manually operated device was used to sort platelets from peripheral blood mononuclear cells (PBMCs). The platelets were collected with a purity of ~ 100%. The purity of PBMCs was enhanced from 0.8 to 10.4% after multiple processes which results in an enrichment ratio of 13.8. During the process of manual hand pumping, the flow fluctuation caused by unstable injection will not influence the sorting performance. Due to its simplicity, this manually operated microfluidic chip is suitable for outfield settings.
Yan, Sheng
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Tan, Say Hwa
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Li, Yuxing
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Tang, Shiyang
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Teo, Adrian J.T.
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Zhang, Jun
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Zhao, Qianbin
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Yuan, Dan
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Sluyter, Ronald
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Nguyen, N. T.
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Li, Weihua
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1 January 2018
Yan, Sheng
1cf2968c-1639-4c47-a90b-481b86c441cb
Tan, Say Hwa
de336252-0c2c-4d54-acb9-46a4a37a6ec6
Li, Yuxing
5e0dfa2f-1857-4c16-b79e-7becedb5eb55
Tang, Shiyang
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Teo, Adrian J.T.
d5cb3c2b-b680-4c8a-9916-5d96972c07e7
Zhang, Jun
a4628c00-1e1c-4729-8d8a-e7619a2c1edf
Zhao, Qianbin
4e956b7f-4fb6-42fa-9a3b-b9a7c3703493
Yuan, Dan
76b9b77e-dda5-4682-8db0-75bfad1d1258
Sluyter, Ronald
9f6fe123-30ee-4cb8-aa24-7e6da60a2bdb
Nguyen, N. T.
1c8ed53f-30d6-4de0-bdd6-e1693d360763
Li, Weihua
e2555036-0e48-425a-afeb-db6ffba5238e
Yan, Sheng, Tan, Say Hwa, Li, Yuxing, Tang, Shiyang, Teo, Adrian J.T., Zhang, Jun, Zhao, Qianbin, Yuan, Dan, Sluyter, Ronald, Nguyen, N. T. and Li, Weihua
(2018)
A portable, hand-powered microfluidic device for sorting of biological particles.
Microfluidics and Nanofluidics, 22 (1), [8].
(doi:10.1007/s10404-017-2026-0).
Abstract
Manually hand-powered portable microfluidic devices are cheap alternatives for point-of-care diagnostics. Currently, on-field tests are limited by the use of bulky syringe pumps, pressure controller and equipment. In this work, we present a manually operated microfluidic device incorporated with a groove-based channel. We show that the device is capable to effectively sort particles/cells by manual hand powering. First, the grooved-based channel with differently sized polystyrene particles was characterized using syringe pumps to study their distributions under various flow rate conditions. Afterward, the particle mixtures were sorted manually using hand power to verify the capability of this device. Finally, the manually operated device was used to sort platelets from peripheral blood mononuclear cells (PBMCs). The platelets were collected with a purity of ~ 100%. The purity of PBMCs was enhanced from 0.8 to 10.4% after multiple processes which results in an enrichment ratio of 13.8. During the process of manual hand pumping, the flow fluctuation caused by unstable injection will not influence the sorting performance. Due to its simplicity, this manually operated microfluidic chip is suitable for outfield settings.
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Published date: 1 January 2018
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Funding Information:
Acknowledgements This work was performed in part at the Queensland Node of the Australian National Fabrication Facility, a company established under the National Collaborative Research Infrastructure Strategy to provide nano-and microfabrication facilities for Australia’s researchers. S.H Tan and N.T.N. gratefully acknowledge the support of the Australian Research Council Linkage Grant (LP150100153), DECRA Fellowship (DE170100600), Griffith University-Peking University Collaboration Grant and Griffith University/Simon Fraser University Collaborative Grant.
Publisher Copyright:
© 2017, Springer-Verlag GmbH Germany, part of Springer Nature.
Identifiers
Local EPrints ID: 481687
URI: http://eprints.soton.ac.uk/id/eprint/481687
ISSN: 1613-4982
PURE UUID: 111002c5-65d4-4b85-b8e8-1d955df32115
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Date deposited: 06 Sep 2023 16:48
Last modified: 18 Mar 2024 04:13
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Contributors
Author:
Sheng Yan
Author:
Say Hwa Tan
Author:
Yuxing Li
Author:
Shiyang Tang
Author:
Adrian J.T. Teo
Author:
Jun Zhang
Author:
Qianbin Zhao
Author:
Dan Yuan
Author:
Ronald Sluyter
Author:
N. T. Nguyen
Author:
Weihua Li
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