A rapid, maskless 3D prototyping for fabrication of capillary circuits: Toward urinary protein detection

Yan, Sheng, Zhu, Yuanqing, Tang, Shi Yang, Li, Yuxing, Zhao, Qianbin, Yuan, Dan, Yun, Guolin, Zhang, Jun, Zhang, Shiwu and Li, Weihua (2018) A rapid, maskless 3D prototyping for fabrication of capillary circuits: Toward urinary protein detection. Electrophoresis, 39 (7), 957-964. (doi:10.1002/elps.201700449).

Abstract

Proteinuria is an established risk marker for progressive renal function loss and patients would significantly benefit from a point-of-care testing. Although extensive work has been done to develop the microfluidic devices for the detection of urinary protein, they need the complicated operation and bulky peripherals. Here, we present a rapid, maskless 3D prototyping for fabrication of capillary fluidic circuits using laser engraving. The capillary circuits can be fabricated in a short amount of time (<10 min) without the requirements of clean-room facilities and photomasks. The advanced capillary components (e.g., trigger valves, retention valves and retention bursting valves) were fabricated, enabling the sequential liquid delivery and sample-reagent mixing. With the integration of smartphone-based detection platform, the microfluidic device can quantify the urinary protein via a colorimetric analysis. By eliminating the bulky and expensive equipment, this smartphone-based detection platform is portable for on-site quantitative detection.

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Contributors

Author: Shi Yang Tang

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