Printed droplet microfluidics for on demand dispensing of picoliter droplets and cells

Cole, Russell H., Tang, Shi Yang, Siltanen, Christian A., Shahi, Payam, Zhang, Jesse Q., Poust, Sean, Gartner, Zev J. and Abate, Adam R. (2017) Printed droplet microfluidics for on demand dispensing of picoliter droplets and cells. Proceedings of the National Academy of Sciences of the United States of America, 114 (33), 8728-8733. (doi:10.1073/pnas.1704020114).

Abstract

Although the elementary unit of biology is the cell, high-throughput methods for the microscale manipulation of cells and reagents are limited. The existing options either are slow, lack single-cell specificity, or use fluid volumes out of scale with those of cells. Here we present printed droplet microfluidics, a technology to dispense picoliter droplets and cells with deterministic control. The core technology is a fluorescence-activated droplet sorter coupled to a specialized substrate that together act as a picoliter droplet and single-cell printer, enabling high-throughput generation of intricate arrays of droplets, cells, and microparticles. Printed droplet microfluidics provides a programmable and robust technology to construct arrays of defined cell and reagent combinations and to integrate multiple measurement modalities together in a single assay.

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Contributors

Author: Shi Yang Tang

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