Lateral trapezoid microfluidic platform for investigating mechanotransduction of cells to spatial shear stress gradients
Lateral trapezoid microfluidic platform for investigating mechanotransduction of cells to spatial shear stress gradients
Microfluidic platforms enable the influence of a variety of chemical and physical gradients on single or multiple cells to be examined and monitored in real-time. Research into chemical gradients has been more prevalent in literature; however, with the interest in mechanotransduction, research investigating the influence of physical gradients, such as force from shear stress, are appearing. Shear stress studies, to date have been focused on using parallel plate flow chambers, or shear stress gradients spanning the length of the microchannel. In this paper, we designed a Trapezoid microchannel, which enables production of a unique lateral spatial shear stress gradient, in order to examine the responses it evokes in cells, using microscopy. We investigated the effect of a lateral spatial shear stress gradient on intracellular calcium signalling in HEK-293-TRPV4 cells. The Trapezoid microchannel was designed to produce a spatial shear stress gradient, spanning the width of the microchannel. Additionally, we investigated the effect of temporal shear stress using the microfluidic platform, to further examine the complexity of the transduction of shear stress forces, which mimic pathological and physiological conditions into cellular signalling. We show that the temporal pattern of shear stress, modulated intracellular calcium signalling, with slower response times (responding to stimulus and reaching maximum Calcium influx), compared to those observed in the absence of temporal shear stress. Our experiments highlight the importance of considering the nature of shear stress under physiological and pathological conditions – with microfluidic platforms providing a useful means to do so.
Discontinuous dielectrophoresis, HEK-293, Intracellular calcium signalling, Microfluidics, Spatial shear stress, Temporal shear stress
963-975
Soffe, Rebecca
c1670df9-4ac4-4abc-8c48-3f6048a4e9fb
Baratchi, Sara
aec99b88-f6af-499d-b71d-2cfbc5fab461
Nasabi, Mahyar
5805bfab-fc16-4873-bc03-d49ffbd8dd19
Tang, Shi Yang
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Boes, Andreas
67d68086-32d6-4178-b127-2ba768c4ba08
McIntyre, Peter
c05d25d3-e22e-4561-a0c5-c6bb14c24955
Mitchell, Arnan
33a18604-17e3-421e-b895-0f420d332cd4
Khoshmanesh, Khashayar
72608a1e-0e41-4360-8065-98d872493aed
3 June 2017
Soffe, Rebecca
c1670df9-4ac4-4abc-8c48-3f6048a4e9fb
Baratchi, Sara
aec99b88-f6af-499d-b71d-2cfbc5fab461
Nasabi, Mahyar
5805bfab-fc16-4873-bc03-d49ffbd8dd19
Tang, Shi Yang
1d0f15c6-2a3e-4bad-a3d8-fc267db93ed4
Boes, Andreas
67d68086-32d6-4178-b127-2ba768c4ba08
McIntyre, Peter
c05d25d3-e22e-4561-a0c5-c6bb14c24955
Mitchell, Arnan
33a18604-17e3-421e-b895-0f420d332cd4
Khoshmanesh, Khashayar
72608a1e-0e41-4360-8065-98d872493aed
Soffe, Rebecca, Baratchi, Sara, Nasabi, Mahyar, Tang, Shi Yang, Boes, Andreas, McIntyre, Peter, Mitchell, Arnan and Khoshmanesh, Khashayar
(2017)
Lateral trapezoid microfluidic platform for investigating mechanotransduction of cells to spatial shear stress gradients.
Sensors and Actuators, B: Chemical, 251, .
(doi:10.1016/j.snb.2017.05.145).
Abstract
Microfluidic platforms enable the influence of a variety of chemical and physical gradients on single or multiple cells to be examined and monitored in real-time. Research into chemical gradients has been more prevalent in literature; however, with the interest in mechanotransduction, research investigating the influence of physical gradients, such as force from shear stress, are appearing. Shear stress studies, to date have been focused on using parallel plate flow chambers, or shear stress gradients spanning the length of the microchannel. In this paper, we designed a Trapezoid microchannel, which enables production of a unique lateral spatial shear stress gradient, in order to examine the responses it evokes in cells, using microscopy. We investigated the effect of a lateral spatial shear stress gradient on intracellular calcium signalling in HEK-293-TRPV4 cells. The Trapezoid microchannel was designed to produce a spatial shear stress gradient, spanning the width of the microchannel. Additionally, we investigated the effect of temporal shear stress using the microfluidic platform, to further examine the complexity of the transduction of shear stress forces, which mimic pathological and physiological conditions into cellular signalling. We show that the temporal pattern of shear stress, modulated intracellular calcium signalling, with slower response times (responding to stimulus and reaching maximum Calcium influx), compared to those observed in the absence of temporal shear stress. Our experiments highlight the importance of considering the nature of shear stress under physiological and pathological conditions – with microfluidic platforms providing a useful means to do so.
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More information
Accepted/In Press date: 24 May 2017
e-pub ahead of print date: 26 May 2017
Published date: 3 June 2017
Keywords:
Discontinuous dielectrophoresis, HEK-293, Intracellular calcium signalling, Microfluidics, Spatial shear stress, Temporal shear stress
Identifiers
Local EPrints ID: 481712
URI: http://eprints.soton.ac.uk/id/eprint/481712
ISSN: 0925-4005
PURE UUID: 2e11b8f2-a3ea-4750-8398-46e0e3b2fb43
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Date deposited: 06 Sep 2023 16:50
Last modified: 18 Mar 2024 04:13
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Contributors
Author:
Rebecca Soffe
Author:
Sara Baratchi
Author:
Mahyar Nasabi
Author:
Shi Yang Tang
Author:
Andreas Boes
Author:
Peter McIntyre
Author:
Arnan Mitchell
Author:
Khashayar Khoshmanesh
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