A CRISPR-Cas12a powered electrochemical sensor based on gold nanoparticles and MXene composite for enhanced nucleic acid detection
A CRISPR-Cas12a powered electrochemical sensor based on gold nanoparticles and MXene composite for enhanced nucleic acid detection
Rapid and accurate detection of nucleic acids plays a critical role in public health, food safety and environmental management. Clustered regularly interspaced short palindromic repeats powered electrochemical sensor (E-CRISPR) is attractive as a point-of-care (POC) testing platform to fulfil this purpose. Yet, bulk noble metals (e.g., gold and platinum) based electrodes that have been widely adopted in E-CRISPR suffer from limited analytical performance and high manufacturing costs. Here, to address this limitation, we present a carbon-based E-CRISPR modified with gold nanoparticles and MXene Ti3C2 (a class of two-dimensional transition metal carbide nanomaterials) that provides highly stable and sensitive transduction of CRISPR-Cas12a trans-cleavage activity. Through systematic evaluation and optimization, our AuNPs/MXene Ti3C2 based E-CRISPR achieve the quantification of human papillomavirus 18 (HPV-18) DNA with a wide range of concentrations from 10 pM to 500 nM with a detection limit of 1.95 pM. We further evaluate the selectivity, degradation resistance and detection capability of the developed sensor during long-term storage. Notably, AuNPs/MXene based E-CRISPR retain more than 70% of initial current after 2 months and deliver reliable analytical results that are unaffected over 42-day storage. Owing to its excellent biofouling-resistant and analytical performance and robust shelf life, our E-CRISPR sensor offers a universal, scalable and low-cost strategy for POC nucleic acid testing.
CRISPR diagnosis, Electrochemical biosensor, Gold nanoparticles, Human papillomavirus detection, MXene TiC
Duan, Haowei
9da40a15-f80e-4458-b873-85718f88e712
Wang, Yizhou
b6fc8ecf-87da-496e-bfe1-338ca8fbec39
Tang, Shi Yang
1d0f15c6-2a3e-4bad-a3d8-fc267db93ed4
Xiao, Ting Hui
d389ce17-675e-4647-9330-7155003220c1
Goda, Keisuke
addd7dfb-c845-4d90-90ab-218e7a11deda
Li, Ming
734c0e4b-d284-491f-9cdc-ac394181bdf9
1 April 2023
Duan, Haowei
9da40a15-f80e-4458-b873-85718f88e712
Wang, Yizhou
b6fc8ecf-87da-496e-bfe1-338ca8fbec39
Tang, Shi Yang
1d0f15c6-2a3e-4bad-a3d8-fc267db93ed4
Xiao, Ting Hui
d389ce17-675e-4647-9330-7155003220c1
Goda, Keisuke
addd7dfb-c845-4d90-90ab-218e7a11deda
Li, Ming
734c0e4b-d284-491f-9cdc-ac394181bdf9
Duan, Haowei, Wang, Yizhou, Tang, Shi Yang, Xiao, Ting Hui, Goda, Keisuke and Li, Ming
(2023)
A CRISPR-Cas12a powered electrochemical sensor based on gold nanoparticles and MXene composite for enhanced nucleic acid detection.
Sensors and Actuators B: Chemical, 380, [133342].
(doi:10.1016/j.snb.2023.133342).
Abstract
Rapid and accurate detection of nucleic acids plays a critical role in public health, food safety and environmental management. Clustered regularly interspaced short palindromic repeats powered electrochemical sensor (E-CRISPR) is attractive as a point-of-care (POC) testing platform to fulfil this purpose. Yet, bulk noble metals (e.g., gold and platinum) based electrodes that have been widely adopted in E-CRISPR suffer from limited analytical performance and high manufacturing costs. Here, to address this limitation, we present a carbon-based E-CRISPR modified with gold nanoparticles and MXene Ti3C2 (a class of two-dimensional transition metal carbide nanomaterials) that provides highly stable and sensitive transduction of CRISPR-Cas12a trans-cleavage activity. Through systematic evaluation and optimization, our AuNPs/MXene Ti3C2 based E-CRISPR achieve the quantification of human papillomavirus 18 (HPV-18) DNA with a wide range of concentrations from 10 pM to 500 nM with a detection limit of 1.95 pM. We further evaluate the selectivity, degradation resistance and detection capability of the developed sensor during long-term storage. Notably, AuNPs/MXene based E-CRISPR retain more than 70% of initial current after 2 months and deliver reliable analytical results that are unaffected over 42-day storage. Owing to its excellent biofouling-resistant and analytical performance and robust shelf life, our E-CRISPR sensor offers a universal, scalable and low-cost strategy for POC nucleic acid testing.
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More information
Accepted/In Press date: 10 January 2023
e-pub ahead of print date: 11 January 2023
Published date: 1 April 2023
Additional Information:
Funding Information:
This research is supported mainly by the Australian Research Council ( ARC ) Discovery Project ( DP200102269 ) and partly by the JSPS Core-to-Core Program . Haowei Duan acknowledges the financial support from iMQRES scholarship at Macquarie University . The authors also thank Dr. Chao Shen and Dr. Sue Lindsay at the Microscopy Unit of Macquarie University for their assistance with FE-SEM and TEM imaging. Fig. 1 and Fig. 3 are created with BioRender.com.
Dr Shi-Yang Tang is currently a Lecturer (Assistant Professor) in the Department of Electronic, Electrical and Systems Engineering at the University of Birmingham, UK. He received his BEng (1st class honours) in Electrical Engineering and Ph.D. in Microelectromechanical Systems (MEMS) from RMIT University, Australia, in 2012 and 2015, respectively. He went to the Pennsylvania State University (PSU) and the University of California, San Francisco (UCSF) to conduct his postdoctoral research in bio-microfluidics. He returned to Australia and joined the University of Wollongong as a Vice-Chancellor's Postdoctoral Research Fellow from 2017 to 2020. He received the Discovery Early Career Researcher Award (DECRA) from the Australian Research Council. Dr Tang's Soft Intelligent Systems research group (https://www.ubham-sis.uk/) conducts research on intelligent microfluidics and liquid metal-enabled electromechanical platforms. He has published more than 90 papers and many of them are in high-impact journals.
Keywords:
CRISPR diagnosis, Electrochemical biosensor, Gold nanoparticles, Human papillomavirus detection, MXene TiC
Identifiers
Local EPrints ID: 481733
URI: http://eprints.soton.ac.uk/id/eprint/481733
ISSN: 0925-4005
PURE UUID: bf7ead3b-fdbb-45c7-b1d4-a99e6588928f
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Date deposited: 06 Sep 2023 16:54
Last modified: 18 Mar 2024 04:13
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Contributors
Author:
Haowei Duan
Author:
Yizhou Wang
Author:
Shi Yang Tang
Author:
Ting Hui Xiao
Author:
Keisuke Goda
Author:
Ming Li
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